Inhibiting Mechanism of Baculovirus p35 Gene to Apoptosis
Inhibiting Mechanism of Baculovirus p35 Gene to Apoptosis出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)
年 卷 期：1998年第3卷第2期
页 面：243-246页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
主 题：apoptosis transformed cells p35 gene baculovirus
摘 要：We transfected Sf9 cells with an expressing vector p35IE1Neo containing antiapoptotic p35 gene and neomycin-resistant gene (as a selection marker). By G418 screening, we got transformed cells that appeared resistant to G418 and picked one clone named Sf9-35. By hybridization in situ, it was found that p35 gene had integrated into the chromosome of Sf9-35 cells; By using actinomycin D treatment and cellular DNA electrophoresis, Sf9-35 cells were found to resist apoptosis induced by infection of vAcΔp35 deleting p35 gene and actinomycin D treatment; And it was also found that apoptosis induced by viral infection and actinomycin D treatment can only be delayed, but can not be stopped in Sf9-35.
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