Elevated miR-33a and miR-224 in steatotic chronic hepatitis Cliver biopsies

作     者：Gabor Lendvai Katalin Jármay Gizella Karácsony Tünde Halász Ilona Kovalszky Kornélia Baghy Tibor Wittmann Zsuzsa Schaff András Kiss 

作者机构：MTA-SE Tumor Progression Research GroupSemmelweis University 1st Department of Medicine University of Szeged 2nd Department of PathologySemmelweis University 1st Department of Pathology and Experimental Cancer Research Semmelweis University 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2014年第20卷第41期

页      面：15343-15350页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Grants from the National Scientific Research Fund No.OTKA K101435 No.K108548 and No.105763 

主　　题：Chronic hepatitis C Steatosis MicroRNA Expression 

摘      要：AIM:To assess the expression of selected microRNAs(miRNA) in hepatitis C,steatotic hepatitis C,noninfected steatotic and normal liver ***:The relative expression levels of miR-21,miR-33 a,miR-96,miR-122,miR-125 b,miR-221 and miR-224 were determined in 76 RNA samples isolated from 18 non-steatotic and 28 steatotic chronic hepatitis C(CHC and CHC-Steatosis,respectively) cases,18 non-infected,steatotic liver biopsies of metabolic origin(Steatosis) and 12 normal formalin-fixed paraffin-embedded liver tissues using TaqMan MicroRNA *** CHC biopsy samples were obtained prior to initiating *** serum biochemical values,which included glucose,triglyceride,cholesterol,alanine aminotransferase(ALT),aspartate aminotransferase(AST),gamma-glutamyl-transferase(GGT),alkaline phosphatase(AP),were obtained and correlated with relative miRNA ***:When compared with control non-infected liver samples,miR-122 and miR-221 levels were reduced in CHC-Steatosis(P 0.03) and in CHC,CHCSteatosis and Steatosis(P 0.01).Alternatively,the expression of miR-33 a and miR-224 were elevated in CHC-Steatosis and Steatosis in comparison to control tissue(P 0.01).The levels of miR-33 a and miR-224 in CHC-Steatosis(P 0.02) and miR-224 in Steatosis(P 0.001) were increased in comparison to CHC *** contrast,the expression of miR-21 did not differ statistically between diseased and normal liver *** of miR-33 a correlated negatively with serum AST and AP levels in Steatosis as well as with necroinflammatory grade in CHC,whereas miR-21 correlated positively with AST in Steatosis and displayed negative correlation with triglyceride level in *** contrast,miRNA levels were not correlated with ALT,GGT,cholesterol levels or fibrosis ***:Differences in miRNA expression were observed between CHC and steatotic CHC,CHC and steatotic liver,but not between steatotic CHC and steatotic liver of metabolic origin.