Expression of cyclin genes in human gastric cancer and in first degree relatives

作     者：于君 Stephan Miehlke Matthias P.A.Ebert Daniel Szokodi B.Wehvnignh Peter Malfertheiner G Ehninger Ekkehard Bayerdoerfer YU Jun;Stephan Miehlke;Matthias P.A.Ebert;Daniel Szokodi;B.Wehvnignh;Peter Malfertheiner;G Ehninger;Ekkehard Bayerdoerffer

作者机构：Department of GastroenterologyFifth HospitalHebei Medical university ShijiazhuangChina Medieal Department I.Technical University HospitalDresden.Gennany Department of GastroenterologyHepatologyand Infectious DiseasesOtto-von-Guericke UniversityMagdeburgGennany 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2002年第115卷第5期

页      面：710-715,151-152页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Adult Aged Aged, 80 and over Cyclin D1 Cyclin E Cyclins Family Health Gastritis Gene Expression Regulation, Neoplastic Helicobacter Infections Helicobacter pylori Humans Middle Aged RNA, Messenger Stomach Stomach Neoplasms 

摘      要：OBJECTIVE: To clarify the role of these cyclins in human gastric cancer. METHODS: 38 gastric cancer patients, 29 first degree relatives of gastric cancer patients, as well as 18 healthy subjects were included. The mRNA expression of cyclins D1, D2, D3 and E in gastric biopsies was evaluated by RT-PCR analysis using specific primers. Histomorphological features such as intestinal metaplasia, atrophy, H. pylori infection and severity of gastritis were determined by the updated Sydney System. RESULTS: Significant mRNA overexpression was found for cyclins D2, D3 and E compared with healthy normal specimen, but cyclin D1 expression was not different between tumor and normal tissues. In addition, cyclin D2 and D3 overexpression was significantly more frequent in first degree relatives than in healthy controls (P