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Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402 involving G2-phase arrest

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Acta Pharmacologica Sinica 2004年第12期25卷 132-138页

作者： Hong-ying CHEN2, Cong-yi ZHENG2, Guo-lin ZOU2, Da-xing XIE3, Jian-ping GONG3 2College of Life Sciences, Wuhan University, Wuhan 430072 3Cancer Research Institute, Tongji Hospital, TongjiMedical College, Huazhong University of Science and Technology, Wuhan 430072, China College of Life Sciences Wuhan University Wuhan 430072 Cancer Research Institute Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430072

AIM: To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402). METHODS: Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis. RESULTS: PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 μmol/Lfor 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h. CONCLUSION: Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest.

关键词： peplomycin Bel-7402 cells apoptosis cyclins cell cycle flow cytometry

调控蛋白cyclins时序性表达与人胚肺成纤维细胞周期进程关系

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中华预防医学杂志 2009年第6期43卷 538-540页

作者：严丽萍陶茂萱中国健康教育中心北京100011

细胞周期关卡阻滞是细胞应对遗传损伤的重要调节机制，也是近年来研究的热点。但连续运转的细胞周期中某一时刻均存在处于各个时相的细胞，G1期占绝大多数，容易掩盖其他细胞周期时相细胞应对遗传损伤的周期阻滞效应。细胞周期同步化可... 详细信息

细胞周期关卡阻滞是细胞应对遗传损伤的重要调节机制，也是近年来研究的热点。但连续运转的细胞周期中某一时刻均存在处于各个时相的细胞，G1期占绝大多数，容易掩盖其他细胞周期时相细胞应对遗传损伤的周期阻滞效应。细胞周期同步化可获得大量处于某一时点的同质性细胞群，是研究遗传损伤后周期调节机制的较好细胞模型。

关键词：细胞周期进程 cyclins 时序性表达调控蛋白成纤维人胚肺细胞周期时相细胞周期同步化

胃肠道恶性肿瘤cyclins表达分型与MDR1及TNM分期的关系

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世界华人消化杂志 2006年第15期14卷 1512-1515页

作者：郭勇覃吉超周毅何小军李伟华谢大兴龚建平华中科技大学同济医学院附属同济医院分子医学中心湖北省武汉市430030

目的:研究胃肠道恶性肿瘤的4种主要cyclins (Cyclin D1,E,A,B1)的表达规律,以此为依据对恶性肿瘤进行分型,并结合肿瘤标本中 MDR1(多药耐药基因1)的表达情况,与肿瘤 TNM分期进行相关性分析．方法:采用流式细胞术分析新鲜手术获取的 62... 详细信息

目的:研究胃肠道恶性肿瘤的4种主要cyclins (Cyclin D1,E,A,B1)的表达规律,以此为依据对恶性肿瘤进行分型,并结合肿瘤标本中 MDR1(多药耐药基因1)的表达情况,与肿瘤 TNM分期进行相关性分析．方法:采用流式细胞术分析新鲜手术获取的 62例肿瘤标本(胃癌32例,结直肠癌30例)的 Cyclin D1,E,A,B1及MDR1表达情况,再记录术后病理报告进行TNM分期．结果:根据4种主要cyclins的表达情况对恶性消化道肿瘤分为Ⅰ-Ⅳ型Cyclin非时相性表达类型,此Ⅰ-Ⅳ型细胞周期类型与TNM分期具有一致性,Kappa系数等于0．599(Pcyclins的表达情况对消化道恶性肿瘤进行的分型具有与TNM分期相同的意义．在消化道肿瘤中,MDR1与肿瘤细胞周期素表达类型有关,对治疗具有指导意义．

关键词： cyclins 细胞周期多药耐药基因1 胃肠道恶性肿瘤 TNM分期流式细胞术

Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera

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Acta Oceanologica Sinica 2020年第10期39卷 27-34页

作者： Juanjuan Yang Dachun Yu Songdong Shen Department of Cell Biology School of Biology and Basic MedicalSoochow UniversitySuzhou 215123China

microRNAs(miRNA)families play a critical role in plant growth,development,and responses to abiotic stress.In this study,we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat stress.The data demonstrated that 184 target genes of Up-miR-843 could be successfully validated.N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes,including cyclin A3 and cyclin L,which were strictly required for cell cycle progression.In addition,U.prolifera with highly expression of Up-miR-843 showed improved biomass,and photosynthesis compared with that under normal growth conditions.Thus,the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes,which further regulated the growth of U.prolifera.

关键词： cyclins nitrogen deprivation microRNA U.prolifera heat stress

Bortezomib effect on E2F and cyclin family members in human hepatocellular carcinoma cell lines

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World Journal of Gastroenterology 2014年第3期20卷 795-803页

作者： Daniele Baiz Barbara Dapas Rossella Farra Bruna Scaggiante Gabriele Pozzato Fabrizio Zanconati Nicola Fiotti Lara Consoloni Sara Chiaretti Gabriele Grassi Department of Life Sciences University of Trieste Wake Forest School of Medicine Department of Cancer Biology and Comprehensive Cancer CenterMedical Center BoulevardWinston SalemNC 27157United States Department of Industrial Engineering and Information Technology University of Trieste Department of ‘‘Scienze Mediche Chirurgiche e della Salute''University of TriesteCattinara Hospital

AIM:To evaluate the effects of the proteasome inhibitor bortezomib(BZB)on E2Fs and related genes in hepatocellular carcinoma(HCC)cells.METHODS:The mRNA levels of the E2F family members(pro-proliferative:E2F1-3 and anti-proliferative:E2F4-8)and of their related genes cyclins and cyclindependent kinases(cdks)were evaluated in two HCC cell lines following a single BZB administration.mRNA levels of the epithelial-mesenchymal transition(EMT)genes were also measured in both cell lines after BZB treatment.The BZB concentration(40 nmol/L)used was chosen to stay well below the maximal amount/cm2recommended for in vivo application,and 2 d incubation was chosen as this time point has been found optimal to detect BZB effects in our previous studies.The HCC cell lines,HepG2 and JHH6,were chosen as they display different phenotypes,hepatocyte-like for HepG2and undifferentiated for JHH6,thus representing an in vitro model of low and high aggressive forms of HCC,respectively.The mRNA levels of the target genes were measured by two-color microarray-based gene expression analysis,performed according to Agilent Technologies protocol and using an Agilent Scan B.For the E2F family members,mRNA levels were quantified by realtime reverse transcription polymerase chain reaction(RT-PCR).Using small interfering RNA’s,the effects of E2F8 depletion on cell number was also evaluated.RESULTS:After BZB treatment,microarray analysis of the undifferentiated JHH6 revealed a significant decrease in the expression of the pro-proliferative E2F member E2F2.Quantitative RT-PCR data were in keeping with the microarray analysis,and showed a significant increase and decrease in E2F8 and E2F2 mRNA levels,respectively.In contrast,BZB treatment of the hepatocyte-like HCC cell line HepG2 had a significant impact on mRNA levels of 5 of the 8 E2F members.In particular,mRNA levels of the pro-proliferative E2F members E2F1,E2F2,and of the anti-proliferative member E2F8,decreased over 80%.Notably,a reduction in E2F8 expression in HepG2 and JHH6 cells following si

关键词： Bortezomib cyclins E2F family Hepatocellular carci

In Silico Identification of Co-transcribed Core Cell Cycle Regulators and Transcription Factors in Arabidopsis

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Journal of Integrative Plant Biology 2007年第8期49卷 1253-1260页

作者： Yixing Wang Ming Yang Department of Botany Oklahoma State University Stillwater OK 74078 USA

Regulatory networks involving transcription factors and core cell cycle regulators are expected to play crucial roles in plant growth and development. In this report, we describe the identification of two groups of co-transcribed core cell cycle regulators and transcription factors via a two-step in silico screening. The core cell cycle regulators include TARDY ASYNCHRONOUS MEIOSIS （CYCA1;2）, CYCB1;1, CYCB2;1, CDKB1;2, and CDKB2;2 while the transcription factors include CURLY LEAF, AINTEGUMENTA, a MYB protein, two Forkhead-associated domain proteins, and a SCARECROW family protein. Promoter analysis revealed a potential web of cross- and self-regulations among the identified proteins. Because one criterion for screening for these genes is that they are predominantly transcribed in young organs but not in mature organs, these genes are likely to be particularly involved in Arabidopsis organ growth.

关键词： Arabidopsis cell cycle cyclin-dependent kinases cyclins transcription factors.

Effects of different concentrations of celecoxib on proliferation and cell cycle of SH-SY-5Y cells and its mechanisms In vitro study

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Neural Regeneration Research 2008年第2期3卷 133-137页

作者： Hu Zhou Wei Wang Hongyi Wang Jinghua Li Department of Hematology Affiliated Hospital of Qingdao Medical College Qingdao University Qingdao 266003 Shandong Province China Department of Clinical Laboratory Qingdao Municipal Hospital Qingdao 266001 Shandong Province China

BACKGROUND： Highly selective cyclooxygenase-2 （COX-2） inhibitors have recently been approved for the treatment of colon cancer, breast cancer, urinary bladder cancer, and skin cancer. For the highly selective COX-2 celecoxib, the mechanism of action for inhibiting neuroblastoma cells is still uncertain. OBJECTIVE： To observe the influence of different celecoxib concentrations on proliferation and cell cycle of SH-SY-5Y cells in vitro and to reveal potential COX-2-independent mechanisms of celecoxib on SH-SY-5Y cells. DESIGN： Controlled experiment. SETTING： Department of Hematology, Affiliated Hospital of the Qingdao Medical College, Qingdao University, Shandong Province. MATERIALS： The study was performed at the Cerebrovascular Disease Institute of Shandong Province and the Laboratory of Molecular Biology, Affiliated Hospital of Qingdao Medical College, Qingdao University between September 2006 and June 2007. The SH-SY-5Y cell line was obtained from the Department of Molecular Biology, Qingdao Medical College, Qingdao University. Celecoxib was obtained from Pfizer Pharmaceuticals LLC, USA. Coulter DNA PREP reagent kit was purchased from Beckman Coulter, Inc. The antibodies against human CyclinD1, P21, and P16 were purchased from Santa Cruz Biotechnology. METHODS： SH-SY-5Y cells were treated with different concentrations of celecoxib （10, 20, 40, and 80 la mol/L） for 48 hours and comprised the experimental groups. The same concentrations of DMSO （dimethyl sulphoxide） treatment for 48 hours served as the control group. MAIN OUTCOME MEASURES： ① Cellular morphology of cells pre-treated and post-treated with celecoxib by inverted microscopy. ② Methabenzthiazuron assay was used to measure cell proliferation. ③ Cell cycle was measured by flow cytometry after incubation with different celecoxib concentrations for 48 hours. ④CyclinD1, P16, and P21 protein expression was detected by Western blot analysis. RESULTS： ① Cellular morphology： The shape of SH-SY-5Y cells pre-treated with celecoxib was a slender, fusiform

关键词： neuroblastoma cyclooxygenase celecoxib cyclins

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cyclins与结直肠癌的研究进展

牡丹江医学院学报 2018年第1期39卷 98-100,104页

作者：施颖超沙瑞华杨浩董爱莲孟敏姜泓羽牡丹江医学院研究生院牡丹江医学院附属红旗医院黑龙江牡丹江157011

细胞周期是细胞生命活动中最重要的过程,而细胞周期蛋白(cyclins)、细胞周期蛋白依赖性激酶(CDKs)及细胞周期蛋白依赖性激酶抑制剂(CKIs)则在细胞周期的调控中起到了关键作用。细胞周期失调与肿瘤的发生存在密切的关联。cyclins表达异... 详细信息

细胞周期是细胞生命活动中最重要的过程,而细胞周期蛋白(cyclins)、细胞周期蛋白依赖性激酶(CDKs)及细胞周期蛋白依赖性激酶抑制剂(CKIs)则在细胞周期的调控中起到了关键作用。细胞周期失调与肿瘤的发生存在密切的关联。cyclins表达异常及调节失控在结直肠癌中尤为常见。阐明cyclins与结直肠癌的关系对结直肠癌的治疗和预后有重要意义。

关键词： cyclins 结直肠癌肿瘤治疗

Curcumin inhibits Proliferation of K562 cells by downreguration of P210bcr/abl-initiated Ras Signal Pathway and cyclins

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Curcumin inhibits Proliferation of K562 cells by downregurat...