Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma:An experimental study
Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma:An experimental study作者机构:Department of Interventional Radiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China. wxyao2001@***
出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))
年 卷 期:2003年第9卷第11期
页 面:2445-2449页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:the National Natural Science Foundation of China No.39770839
主 题:肝细胞瘤 血管内皮生长因子 血管生成 基质纤维原生长因子 发病机制 动物实验
摘 要:AIM: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive *** example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF)and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted ***: Male Wistar rats were inoculated with Walker256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor Ⅷ, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density(MVD) was counted in endothelial cells with positive factor Ⅷ. Their expression levels were analyzed in conjunction with the pathologic ***: While a smaller tumor volume was found in TACE group (F=37.818, P0.001), no statistical differences between MVD and expression of VEGF and b-FGF were found among the 3 groups. MVD of the control group, chemotherapy group and chemoemoblization group was 80.84±24.24,83.05±20.29 and 85.20±23.91 (F=0.193, P=0.873),respectively. The positive expression of VEGF and b-FGF was 75 %, 75 % , 85 % (X2=0.449, P=0.799) and 30 %,25 %, 30 % (X2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P0.001).CONCLUSION: There has been little influence of lipiodol chemoembolization on the formation of tumor angiogenesis,but the development of neovascularization and expressionof VEGF play important roles