Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma:An experimental study

作     者：Xin Li Gan-Sheng Feng Chuan-Sheng Zheng Chen-Kai Zhuo Xi Liu, Department of Interventional Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China 

作者机构：Department of Interventional Radiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China. wxyao2001@*** 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2003年第9卷第11期

页      面：2445-2449页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China No.39770839 

主　　题：肝细胞瘤 血管内皮生长因子 血管生成 基质纤维原生长因子 发病机制 动物实验 

摘      要：AIM: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive *** example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF)and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted ***: Male Wistar rats were inoculated with Walker256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor Ⅷ, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density(MVD) was counted in endothelial cells with positive factor Ⅷ. Their expression levels were analyzed in conjunction with the pathologic ***: While a smaller tumor volume was found in TACE group (F=37.818, P0.001), no statistical differences between MVD and expression of VEGF and b-FGF were found among the 3 groups. MVD of the control group, chemotherapy group and chemoemoblization group was 80.84±24.24,83.05±20.29 and 85.20±23.91 (F=0.193, P=0.873),respectively. The positive expression of VEGF and b-FGF was 75 %, 75 % , 85 % (X2=0.449, P=0.799) and 30 %,25 %, 30 % (X2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P0.001).CONCLUSION: There has been little influence of lipiodol chemoembolization on the formation of tumor angiogenesis,but the development of neovascularization and expressionof VEGF play important roles