Decellularized aorta of fetal pigs as a potential scaffold for small diameter tissue engineered vascular graft

作     者：LIU Guo-feng HE Zhi-juan YANG Da-ping HAN Xue-feng GUO Tie-fang HAO Chen-guang MA Hui NIE Chun-lei 

作者机构：Department of Plastic Surgery Second Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150086 China Department of Obstetrics and Gynecology First Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150010 China Department of Plastic Surgery Beijing Chaoyang Hospital Beijing 100020 China Department of Orthopaedics Heilongjiang Provincial Hospital Harbin Heilongjiang 150036 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2008年第121卷第15期

页      面：1398-1406页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：This work was supported by a grant from the National Natural Science Foundation of China (No. 30325042) 

主　　题：tissue engineering blood vessels extracellular matrix calcium 

摘      要：Background For cardiovascular tissue engineering, acellularized biomaterials from pig have been widely investigated. Our purpose was to study mechanical properties and biocompatibility of decellularized aorta of fetal pigs （DAFP） to determine its potential as scaffold for small diameter tissue engineered vascular graft. Methods Descending aorta of fetal pigs was removed cells using trypsin, ribonuclease and desoxyribonuclease. Mechanical properties of DAFP were evaluated by tensile stress-strain and burst pressure analysis. Assessment of cell adhesion and compatibility was conducted by seeding porcine aortic endothelial cells. To evaluate biocompatibility in vivo DAFP was implanted subcutaneously into adult male Sprague Dawley rats for 2, 4 and 8 weeks. Results Histochemistry and scanning electron microscopy examination of DAFP revealed well-preserved extracellular matrix proteins and porous three-dimensional structures. Compared with fresh aorta, DAFP had similar ultimate tensile strength, axial compliance and burst pressure. Cell culture studies in vitro showed that porcine aortic endothelial cells adhered and proliferated on the surfaces of DAFP with excellent cell viability. Subdermal implantation demonstrated that the DAFP did not show almost any immunological reaction and exhibited minimal calcification during the whole follow-up period. Conclusion The DAFP has the potential to serve as scaffolds for small diameter tissue engineered vascular graft.