Study on the Technical Parameters for Estimating HIV-1 Incidence by Using a Recombinant Antigen-based Capture Enzyme Immunoassay—China

作     者：Wenli Liang Jibao Wang Hongxia Yan Xinhui Zhang Dorjiwangmo Dongmin Li Xing Duan Hao Wu Yinyin Wang Li Bai Jian Sun Mengjie Han Yikui Wang Bin Su Min Wang Tashibazong Wenge Xing Cui Zhang Ruijuan Qiao Maofeng Qiu 

作者机构：National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Department of AIDS/STD Control and PreventionDehong Center for Disease Control and PreventionMangshi CountyDehong Dai and Jingpo Autonomous PrefectureYunnan ProvinceChina Beijing Key Laboratory for HIV/AIDS ResearchBeijing Youan HospitalCapital Medical UniversityBeijingChina Institute of Infectious Diseases Prevention and TreatmentGuizhou Provincial Center for Disease Control and PreventionGuiyang CityGuizhou ProvinceChina Department of AIDS Control and PreventionXizang Autonomous Region Center for Disease Control and PreventionLhasa CityXizang Autonomous RegionChina Experimental CenterGuizhou Provincial Center for Disease Control and PreventionGuiyang CityGuizhou ProvinceChina Department of AIDS Control and PreventionGansu Provincial Center for Disease Control and PreventionLanzhou CityGansu ProvinceChina Laboratory DepartmentLiupanshui Center for Disease Control and PreventionLiupanshui CityGuizhou ProvinceChina 

出 版 物：《China CDC weekly》 (中国疾病预防控制中心周报（英文）)

年 卷 期：2024年第6卷第48期

页      面：1278-1282页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：Supported by the National Science and Technology Major Project of the 13th Five-year Plan(grants 2017ZX10201101-002-003 and 2018ZX10721102-003-002) 

主　　题：Antigen exceeding false 

摘      要：Introduction:A novel recombinant antigen-based capture enzyme immunoassay(RAg-CEIA)was optimized and used to determine technical parameters for estimating human immunodeficiency virus type 1(HIV-1)incidence in ***:We employed orthogonal experimental design to optimize RAg-CEIA by adjusting raw material dilution *** assay was used to measure normalized optical density(ODn)values in 171 longitudinal plasma specimens from 51 HIV-1 seroconverting individuals,plotted against estimated days *** determined the optimal ODn threshold value for differentiating recent from long-term infections and calculated the mean duration of recent infection(MDRI)for incidence *** false recent rate(FRR)was determined using 481 HIV-1 antibody-positive specimens with infection durations exceeding twice the ***:Optimal RAg-CEIA parameters were established with a raw material dilution ratio of 1/12 for calibrator preparation and an enzyme conjugate titer of 1:*** values demonstrated consistent temporal increases across HIV-1 seroconverting individuals,though with notable kinetic heterogeneity in individual *** optimal ODn threshold value of 0.8 for distinguishing recent from long-term infections corresponded to an MDRI of 205 days and an FRR of 4.78%.Conclusions:The optimized RAg-CEIA effectively differentiates recent from long-term HIV-1 infections at the population level,enabling reliable HIV-1 incidence estimation in China.