A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis

作     者：Michaela Králová Ivona Kubalová Jakub Hajný Karolina Kubiasová Karolína Vagaská Zengxiang Ge Michelle Gallei Hana Semerádová Anna Kuchařová Martin Hönig Aline Monzer Martin Kovačik JiříFriml Ondřej Novák Eva Benková Yoshihisa Ikeda David Zalabák Michaela Králová;Ivona Kubalová;Jakub Hajný;Karolina Kubiasová;Karolína Vagaská;Zengxiang Ge;Michelle Gallei;Hana Semerádová;Anna Kuchařová;Martin Hönig;Aline Monzer;Martin Kovačik;Jiří Friml;Ondřej Novák;Eva Benková;Yoshihisa Ikeda;David Zalabák

作者机构：Laboratory of Growth RegulatorsFaculty of Science of PalackýUniversity and Institute of Experimental Botany AS CRŠlechtitelů2778371 OlomoucCzech Republic Department of Molecular BiologyCentre of the Region Hanáfor Biotechnological and Agricultural ResearchFaculty of SciencePalackýUniversityŠlechtitelů2778371 OlomoucCzech Republic Institute of Science and Technology Austria(ISTA)3400 KlosterneuburgAustria Department of Chemical BiologyFaculty of SciencePalackýUniversityŠlechtitelů2778371 OlomoucCzech Republic Institute of Experimental Botany of the Czech Academy of SciencesCentre of Plant Structural and Functional GenomicsŠlechtitelů3177900 OlomoucCzech Republic These authors contributed equally to this article. 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2024年第17卷第12期

页      面：1850-1865页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Czech Science Foundation via project 23-07363S(to D.Z.,J.H.,K.V.,and O.N.) Scientific Service Units(SSU)of ISTA through resources provided by the Imaging and Optics Facility and the Life Science Facility 

主　　题：splicing Arabidopsis decoy 

摘      要：Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of ***,a class of plant hormones,regulate cell division and meristem *** cytokinin signaling pathway is well established in the model plant *** negative feedback mechanisms,tightly controlling cytokinin signaling output,have been described *** this study,we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1.A novel splicing variant named CRE1int7 results from seventh intron retention,introducing a premature termination codon in the *** showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal ***1int7 can bind cytokinin but cannot activate the downstream *** present a novel negative feedback mechanism of the cytokinin signaling pathway,facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand *** proper plant growth and development requires precise control of the cytokinin signaling pathway at several ***1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis.