Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers

作     者：Xiao-Ya Guo Zhi-Yu Yang Hua-Pan Fang Dan-Hua Zhou Xuan Pang Hua-Yu Tian Xue-Si Chen 

作者机构：Changchun Institute of Applied Chemistry Chinese Academy of Sciences School of Applied Chemistry and Engineering University of Science and Technology of China State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical Engineering Xiamen University Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) 

出 版 物：《Chinese Journal of Polymer Science》 (高分子科学(英文版))

年 卷 期：2024年第11期

页      面：1699-1709页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：financially supported by National Key Research and Development Program of China (No.2021YFB3800900) the National Natural Science Foundation of China (Nos.51925305,51833010 and 52203183) Natural Science Foundation of Xiamen,China (No.3502Z202371004) Fundamental Research Funds for the Central Universities (No.20720230004) the talent cultivation project Funds for the Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (No.HRTP-52) 

摘      要：Polyethyleneimine （PEI）,as a widely used polymer material in the field of gene delivery,has been extensively studied for modification and shielding to reduce its ***,research aimed at preparing degradable PEI is *** this work,the hydrogen peroxide（H2O2） oxidation method was used to introduce degradable amide groups in the PEI and a series of oxidized PEI22k （oxPEI22k） with different degrees of oxidation were synthesized by regulating the dosage of *** relationship between the oxidation degree of oxPEI22k and the gene transfection efficiency of oxPEI22k was studied in detail,confirming that the oxPEI22k with oxidation degrees of 16.7%and 28.6%achieved improved transfection efficiency compared to unmodified *** oxPEI22k also proved reduced cytotoxicity and improved ***,this strategy was extended to the synthesis of low-molecular-weight *** oxPEI1.8k with suitable oxidation degree also achieved improved transfection efficiency and reduced *** brief,this work provided high-efficiency and low-cytotoxicity degradable gene delivery carriers by regulating the oxidation degree of PEI,which was of great significance for promoting clinical applications of PEI.