Impairment of Triptolide on Liver Mitochondria in Isolated Liver Mitochondria and HL7702 Cell Line

作     者：傅强 江振洲 张陆勇 

作者机构：Jiangsu Center for Drug ScreeningChina Pharmaceutical University Department of Pharmacology for Chinese Materia MedicaChina Pharmaceutical University Key Laboratory of Drug Quality Control and Pharmacovigilance China Pharmaceutical UniversityMinistry of Education 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2013年第19卷第9期

页      面：683-688页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Special Fund of Traditional Chinese Medicine for Public Interest Research from the Ministry of Finance of China(No.200707008) Mega-projects of Science Research for the 11th Five-Year Plan(No.2009ZX09302-002) 

主　　题：triptolide mitochondria hepatotoxicity 

摘      要：Objective： To observe the impairing effects of triptolide on liver mitochondria in isolated rat-liver mitochondria and human normal liver HL7702 cell line. Methods： Rat-liver mitochondria were isolated from adult female Sprague-Dawley （SD） rats. Liver mitochondria were incubated with 0, 1.25, 2.5, 5 and 10 pmol/L triptolide for detecting mitochondrial swelling and with 0, 2.5, 5 and 10 μmol/L triptolide for mitochondrial permeability transition pore （MPTP） activity, rvlitochondrial swelling was estimated by measuring the apparent absorbance change during 600 s in the mitochondrial suspensions at 520 nm with a mitochondrial swelling examining kit. The effect of triptolide on MPTP was determined with a fluorescence detection kit by detecting the fluorescence intensity at an excitation wavelength of 488 nm emitted at 527 nm. Human normal liver HL7702 cells were treated without or with 0.02, 0.1 and 0.5μmol/L triptolide for 24 h for analyzing mitochondrial transmembrane potential （μm） and reactive oxygen species （ROS）. △ψm was measured using the fluorescent probe 5,5＇,6,6＇-tetrachloro-1,1＇,3,3＇-tetraethylbenzimidazolylcarbocyanine iodide （JC-1）. ROS was measured using fluorescent probe 2＇,7＇-dichlorofluorescin diacetate （DCFH-DA）. The cells were harvested and dyed with JC-1 and DCFH-DA, and analyzed by flow cytometry, respectively. Results： Incubation of isolated mitochondria with triptolide results in swollen mitochondda in a concentration-dependent manner. Moreover, triptolide significantly activated mitochondrial permeability transition at 5 and 10 μmol/L （P〈0.05 and P〈0.01）. When HL7702 cells were exposed to a various concentration triptolide for 24 h, mitochondrial membrane depolarization and increase of ROS were caused by triptolide in a concentration-dependent manner. Triptolide significantly induced the mitochondrial membrane depolarization at 0.1 and 0.5 μmol/L （P〈0.05 and P〈0.01） and the increase of ROS at 0.1 and 0.5 μmol/L （P〈0.05 and P〈0.01）. Conclusion： Tr