检索结果-南通市图书馆

triptolide reduces prostate size and androgen level on testosterone-induced benign prostatic hyperplasia in Sprague Dawley rats

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第5期15卷 341-346页

作者： WANG Yu-Rong XU Yuan JIANG Zhen-Zhou ZHANG Lu-Yong WANG Tao State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing 210009 China Center for Molecular Metabolism School of Environmental and Biological Engineering Nanjing University of Science andTechnology Nanjing 210014 China Jiangsu Key Laboratory of Drug Screening and Jiangsu Center for Pharmacodynamics Research and Evaluation China Pharmaceutical University Nanjing 210009 China Jiangsu Key Laboratory of TCM Evaluation and Translational Research China Pharmaceutical University Nanjing 210009China

Benign prostatic hyperplasia(BPH) is an age-related disease of unknown etiology, characterized by prostatic enlargement coincident with distinct alterations in tissue histology. In the present study, we investigated whether triptolide can prevent testosterone-induced prostatic hyperplasia in rats. Castration was performed via the scrotal route after urethane aesthesia. BPH was induced in experimental groups by daily subcutaneous injections of testosterone propionate(TP) for two weeks. triptolide was administered daily by oral gavage at a dose of 100 and 50 μg×kg^(-1) for 2 weeks, along with the TP injections. On day 14, the animals were humanely killed by cervical dislocation after aesthesia. Prostates were excised, weighed, and used for histological studies. Testosterone and dihydrotestosterone(DHT) levels in serum and prostate were measured. The results showed that triptolide significantly reduced the prostate weight, and the testosterone and DHT levels in both the serum and prostate. Histopathological examination also showed that triptolide treatment suppressed TP-induced prostatic hyperplasia. In conclusion, triptolide effectively inhibits the development of BPH induced by testosterone in a rat model.

关键词： triptolide Benign prostatic hyperplasia Androgen

triptolide inhibits TGF-β-induced matrix contraction and fibronectin production mediated by human Tenon fibroblasts

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2018年第7期11卷 1108-1113页

作者： Yang Liu Ping-Ping Liu Lei Liu Xiao-Shuo Zheng Hui Zheng Cheng-Cheng Yang Ci-Ren Luobu Ye Liu Department of Ophthalmology the Fifth Affiliated Hospital Sun Yat-sen University Department of Ophthalmology the First Hospital of Jilin University Department of Pathology the Fifth Affiliated Hospital Sun Yat-sen University

AIM： To determine if triptolide influences the contractility and fibronectin production in human Tenon fibroblasts（HTFs）. METHODS： HTFs were cultured in type I collagen gels with or without transforming growth factor beta（TGF-β） and/or triptolide. The diameter of the collagen gel was used to measure contraction. Immunoblot analysis was used to quantify myosin light chain（MLC） phosphorylation and integrin expression. Laser confocal fluorescence microscopy was used to monitor the formation of actin stress fibers. Fibronectin production was measured with an enzyme immunoassay. RESULTS： triptolide inhibition of contraction in TGF-β-induced collagen gel mediated by HTFs was dosedependent and statistically significant at 3 nmol/L（P〈0.05） and maximal at 30 nmol/L and significantly time dependent at 2 d（P〈0.05）. triptolide reduced TGF-β-induced expression of integrins α5 and β1, phosphorylation of MLC, and formation of stress fibers in HTFs. Furthermore, the inhibition of triptolide on the attenuated TGF-β-induced production of fibronectin by HTFs was concentration-dependent and significant at 1 nmol/L（P〈0.05） and maximal at 30 nmol/L. CONCLUSION： triptolide suppress the contractility of HTFs induced by TGF-β and the production of fibronectin by these cells. It is promising that triptolide treatment may possibly inhibit scar formation after glaucoma filtration surgery.

关键词： Tenon fibroblast triptolide transforming growth factor β wound healing fibronectin

A pH-sensitive supramolecular nanosystem with chlorin e6 and triptolide co-delivery for chemo-photodynamic combination therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Pharmaceutical Sciences 2022年第2期17卷 206-218页

作者： Yihan Wu Jingjing Li Xuemei Zhong Jinfeng Shi Yanfen Cheng Chenglin He Jiaxin Li Liang Zou Chaomei Fu Meiwan Chen Jinming Zhang Huile Gao School of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China Department of Pharmacology and Pharmacy The University of Hong KongHong Kong SARChina School of Food and Biological Engineering Chengdu UniversityChengdu 610106China Institute of Chinese Medical Sciences University of MacaoMacaoChina West China School of Pharmacy Sichuan UniversityChengdu 610041China

The combination of Ce6,an acknowledged photosensitizer,and TPL,a natural anticancer agent,has been demonstrated as a useful strategy to reinforce the tumor growth suppression,as well as decrease the systemic side effects compared with their monotherapy.However,in view of the optimal chemo-photodynamic combination efficiency,there is still short of the feasible nanovehicle to steadily co-deliver Ce6 and TPL,and stimuli-responsively burst release drugs in tumor site.Herein,we described the synergistic antitumor performance of a pH-sensitive supramolecular nanosystem,mediated by the host–guest complexing betweenβ-CD and acid pH-responsive amphiphilic co-polymer mPEG-PBAE-mPEG,showing the shell–core structural micelles with the tightβ-CD layer coating.Both Ce6 and TPLwere facilely co-loaded into the spherical supramolecular NPs(TPL+Ce6/NPs)by one-step nanoprecipitation method,with an ideal particle size(156.0 nm),acid pH-responsive drug release profile,and enhanced cellular internalization capacity.In view of the combination benefit of photodynamic therapy and chemotherapy,as well as co-encapsulation in the fabricated pH-sensitive supramolecular NPs,TPL+Ce6/NPs exhibited significant efficacy to suppress cellular proliferation,boost ROS level,lower MMP,and promote cellular apoptosis in vitro.Particularly,fluorescence imaging revealed that TPL+Ce6/NPs preferentially accumulated in the tumor tissue area,with higher intensity than that of free Ce6.As expected,upon 650-nm laser irradiation,TPL+Ce6/NPs exhibited a cascade of amplified synergistic chemo-photodynamic therapeutic benefits to suppress tumor progression in both hepatoma H22 tumor-bearingmice and B16 tumor-bearingmice.More importantly,lower systemic toxicitywas found in the tumor-bearingmice treated with TPL+Ce6/NPs.Overall,the designed supramolecular TPL+Ce6/NPs provided a promising alternative approach for chemo-photodynamic therapy in tumor treatment.

关键词： triptolide Chemo-photodynamic pH-sensitive supramolecular Nanosystem Co-delivery

Injectable thermo-responsive nano-hydrogel loading triptolide for the anti-breast cancer enhancement via localized treatment based on “two strikes” effects

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2020年第11期10卷 2227-2245页

作者： Yaoyao Luo Jingjing Li Yichen Hu Fei Gao George Pak-Heng Leung Funeng Geng Chaomei Fu Jinming Zhang College of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China Department of Pharmacology and Pharmacy University of Hong KongHongKong 999077China College of Pharmacy and Biological Engineering Chengdu UniversityChengdu 610100China Sichuan Key Laboratory of Medical American Cockroach Chengdu 615000China

The clinical application of triptolide(TPL)in tumor therapy has been greatly limited by its toxicity and inefficient delivery.Herein,a localized and sustained-release thermo-sensitive hydrogel was developed for the intra-tumor administration of TPL.Based on the amphiphilic structure of poly(N-isopropylacrylamide-co-acrylic acid)-g-F68 copolymer,it was able to form nano-micelles to efficiently encapsulate TPL,and then turn into a hydrogel at 37C.TPL@nano-gel exhibited a sustained drug release profile in vitro and a stronger anticancer effect caused by"two strikes".The"first strike"was its enhanced cytotoxicity compared to free TPL,due to the enhanced pro-apoptosis effect observed in both MDA-MB-231 and MCF-7 cells caused by the regulation of endogenous mitochondrial pathways.Furthermore,TPL@nano-gel exhibited a"second-strike"through its anti-angiogenesis capabilities mediated through VEGFR-2 signaling inhibition.As expected,after intra-tumoral injection at a 0.45 mg/kg TPL-equivalent dose three times over 14 days in 4 T1 tumor-bearing mice,TPL@nano-gel led to lower systemic toxicity and higher antitumor efficacy compared to multiple injections of TPL.In this regard,these findings indicate that this injectable thermo-responsive hydrogel carries great potential for TPL as a safe and effective cancer therapy.

关键词： Thermo-responsive hydrogel triptolide Tumor local treatment Breast cancer Anti-angiogenesis Pro-apoptosis Micelle Survival rate

Silencing ribosomal protein L4 enhances the inhibitory effects of triptolide on non-small cell lung cancer cells by disrupting the mouse double minute 2 protein–P53 tumor suppressor pathway

在线全文

学校读者我要写书评

暂无评论

生物细胞(英文) 2023年第9期47卷 2009-2026页

作者： NAN TANG YAJING ZHAN JIAYAN MAO ANKANG YIN WEI WANG JUAN WANG School of Medical Technology and Information Engineering Zhejiang Chinese Medical UniversityHangzhou310053China Zhejiang Chinese Medical University Hangzhou310053China Department of Clinical Laboratory Key Laboratory of Cancer Prevention and Therapy Combining Traditional Chinese and Western Medicine of Zhejiang ProvinceZhejiang Academy of Traditional Chinese MedicineTongde Hospital of Zhejiang ProvinceHangzhou310012China

Non-small cell lung cancer(NSCLC)is a malignant tumor with high incidence worldwide.triptolide(TP),extracted from Tripterygium wilfordii Hook F,exhibits potent broad-spectrum antitumor activity.Although some mechanisms through which TP inhibits NSCLC are well understood,those that involve ribosomal proteins remain yet to be understood.In this study,the transcriptome and proteome were integrated and analyzed.Our data indicated ribosomal protein L4(RPL4)to be a core hub protein in the protein-protein interaction network.RPL4 is overexpressed in NSCLC tissues and cells.Transfection with siRPL4 or TP treatment alone arrested the cell cycle in the G1 phase,induced cell apoptosis,and repressed cell invasion.Compared to treating cells with TP alone or siRPL4,treating them with siRPL4–TP enhanced the inhibition of NSCLC cells.Reduced RPL4 expression reinforced the inhibitory effects of TP on NSCLC cells by disrupting the MDM2-P53 pathway and by altering the expression of PARP1/Snail/cyclin D1.In vivo assays verified that TP induced cell apoptosis and reduced RPL4 expression in xenografts.These findings provide clues to facilitate the development of effective TP-based therapeutic strategies to kill NSCLC cells.

关键词： NSCLC RPL4 triptolide Proteomics Transcriptome

Synergetic delivery of triptolide and Ce6 with light-activatable liposomes for efficient hepatocellular carcinoma therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第7期11卷 2004-2015页

作者： Ling Yu Zhenjie Wang Zhuomao Mo Binhua Zou Yuanyuan Yang Rui Sun Wen Ma Meng Yu Shijun Zhang Zhiqiang Yu Department of Traditional Chinese Medicine the First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China The People’s Hospital of Gaozhou Maoming 525200China School of Pharmaceutical Sciences Guangdong Provincial Key Laboratory of New Drug ScreeningSouthern Medical UniversityGuangzhou 510515China

Hepatocellular carcinoma(HCC)has been known as the second common leading cancer worldwide,as it responds poorly to both chemotherapy and medication.triptolide(TP),a diterpenoid triepoxide,is a promising treatment agent for its effective anticancer effect on multiple cancers including HCC.However,its clinical application has been limited owing to its severe systemic toxicities,low solubility,and fast elimination in the body.Therefore,to overcome the above obstacles,photo-activatable liposomes(LP)integrated with both photosensitizer Ce6 and chemotherapeutic drug TP(TP/Ce6-LP)was designed in the pursuit of controlled drug release and synergetic photodynamic therapy in HCC therapy.The TP encapsulated in liposomes accumulated to the tumor site due to the enhanced permeability and retention(EPR)effect.Under laser irradiation,the photosensitizer Ce6 generated reactive oxygen species(ROS)and further oxidized the unsaturated phospholipids.In this way,the liposomes were destroyed to release TP.TP/Ce6-LP with NIR laser irradiation(TP/Ce6-LP+L)showed the best antitumor effect both in vitro and in vivo on a patient derived tumor xenograft of HCC(PDXHCC).TP/Ce6-LP significantly reduced the side effects of TP.Furthermore,TP/Ce6-LP+L induced apoptosis through a caspase-3/PARP signaling pathway.Overall,TP/Ce6-LP+L is a novel potential treatment option in halting HCC progression with attenuated toxicity.

关键词： Hepatocellular carcinoma Synergetic delivery triptolide Ce6 Photo-activatable liposomes Photosensitizer Process of photodynamic therapy PDX model

Altered integrity of hepatocyte tight junctions in rats with triptolide-induced cholestasis

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2021年第3期19卷 188-194页

作者： HUANG Shan LIU Li MEI Hui-Fang ZHANG Qian-Wen ZHANG Xi XU Xiao-Ting WANG Xin-Zhi HUANG Xin WANG Tao JIANG Zhen-Zhou ZHANG Lu-Yong SUN Li-Xin Jiangsu Key Laboratory of Drug Screening China Pharmaceutical UniversityNanjing 210009China Center for Drug Research and Development Guangdong Pharmaceutical UniversityGuangzhou 510006China Jiangsu Center for Pharmacodynamics Research and Evaluation China Pharmaceutical UniversityNanjing 210009China Changde Vocational Technology College Changde 415000China

triptolide(TP),an active component of Tripterygium wilfordii Hook.f.(TWHF),has been widely used for centuries as a traditional Chinese medicine.However,the clinical application of TP has been restricted due to multitarget toxicity,such as hepatotoxicity.In this study,28 days of oral TP administration(100,200,or 400μg·kg^(-1)·d^(-1))induced the occurrence of cholestasis in female Wistar rats,as evidenced by increased serum levels ofγ-glutamyl transpeptidase(γ-GGT),alkaline phosphatase(ALP)and hepatic total bile acids(TBAs).In addition,the heptocyte polarity associated with the strcture of tight junctions(TJs)was disrupted in both rats and sandwich-cultured primary hepatocytes.Immunoblotting revealed decreased expression of the TJ-associated proteins occludin,claudin^(-1),and zonula occludens protein(ZO^(-1)),and downregulated m RNA levels of these TJs was also detected by real-time PCR.An immunofluorescence analysis showed abnormal subcellular localization of occludin,claudin^(-1) and ZO^(-1),which was also confirmed by transmission electron microscopy.Moreover,the concentration of FITC-dextran,a marker of paracellular penetration,was found to increase rapidly in bile increased rapidly(within 6 minutes)after treatment with TP,which indicated the functional impairment of TJs.Taken together,these results suggest that the administration of TP for 28 consecutive days to rats could induce cholestatic injury in the liver,and the increased paracellular permeability might play an important role in these pathological changes.

关键词： triptolide Cholestatic injury Tight junction Sandwich-cultured primary hepatocytes

Superior in vitro anticancer effect of biomimetic paclitaxel and triptolide co-delivery system in gastric cancer

在线全文

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2021年第4期35卷 327-338页

作者： Siwan Wang Hui Jiang Jia Wang Haisi Wu Ting Wu Mengnan Ni Qianqian Zhao You Ji Ziting Zhang Chunming Tang Huae Xu Department of Pharmaceutics School of PharmacyNanjing Medical UniversityNanjingJiangsu 211166China Jiangsu Key Laboratory of Molecular and Translational Cancer Research Affiliated Cancer Hospital of Nanjing Medical UniversityJiangsu Cancer HospitalJiangsu Institute of Cancer ResearchNanjingJiangsu 210009China Department of Geriatric Gastroenterology the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China

As a well-known anticancer drug,paclitaxel(PTX),a first-line chemotherapeutic agent,remains unsatisfactory for gastric cancer therapy.It is reported that triptolide(TPL)could enhance the anti-gastric cancer effect of PTX.Considering the poor solubility of both drugs,we developed a red blood cell membrane-biomimetic nanosystem,an emerging tool in drug delivery,to co-load paclitaxel and triptolide(red blood cell membrane coated PTX and TPL co-loaded poly(lactic-co-glycolic acid)[PLGA]nanoparticles,RP(P/T)).The successful preparation was confirmed in terms of particle size,morphology,and surface markers assays.This biomimetic system could prolong circulation and escape immune surveillance.And these properties were verified by stability,in vitro drug release,and cellular uptake assays.Moreover,the MTT and colony formation assays demonstrated the superior anti-proliferation effect of the RP(P/T)to free drugs.The enhanced antitumor effects of RP(P/T)on migration and invasion were also evaluated by wound-healing and transwell assays.Overall,the bionic co-delivery nanoplatform with improved efficacy in vitro is a promising therapy for gastric cancer.

关键词： paclitaxel triptolide red-blood-cell membrane gastric cancer

Differential expression of the TwHMGS gene and its effect on triptolide biosynthesis in Tripterygium wilfordii

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2019年第8期17卷 575-584页

作者： TONG Yu-Ru ZHANG Yi-Feng ZHAO Yu-Jun HU Tian-Yuan WANG Jia-Dian HUANG Lu-Qi GAO Wei School of Pharmacy Traditional Chinese Materia Medica Shenyang Pharmaceutical University Shenyang 110016 China National Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700 China School of Pharmaceutical Science Capital Medical University Beijing 100069 China School of Traditional Chinese Medicine Capital Medical University Beijing 100069 China Advanced Innovation Center for Human Brain Protection Capital Medical University Beijing 100069 China

3-Hydroxy-3-methylglutaryl-CoA synthase(HMGS) is the first committed enzyme in the MVA pathway and involved in the biosynthesis of terpenes in Tripterygium wilfordii. The full-length cDNA and a 515 bp RNAi target fragment of TwHMGS were ligated into the p H7 WG2 D and p K7 GWIWG2 D vectors to respectively overexpress and silence, Tw HMGS was overexpressed and silenced in T. wilfordii suspension cells using biolistic-gun mediated transformation, which resulted in 2-fold increase and a drop to70% in the expression level compared to cells with empty vector controls. During Tw HMGS overexpression, the expression of TwHMGR, TwDXR and TwTPS7 v2 was significantly upregulated to the control. In the RNAi group, the expression of Tw HMGR,TwDXS, TwDXR and TwMCT visibly displayed downregulation to the control. The cells with TwHMGS overexpressed produced twice higher than the control value. These results proved that differential expression of Tw HMGS determined the production of triptolide in T.wilfordii and laterally caused different trends of relative gene expression in the terpene biosynthetic pathway. Finally, the substrate acetyl-Co A was docked into the active site of TwHMGS, suggesting the key residues including His247, Lys256 and Arg296 undergo electrostatic or H-bond interactions with acetyl-CoA.

关键词： Overexpression RNAi HMGS triptolide Acetyl-CoA