Occurrence and molecular characterization of Potato spindle tuber viroid(PSTVd) isolates from potato plants in North China

作     者：QIU Cai-ling ZHANG Zhi-xiang LI Shi-fang BAI Yan-ju LIU Shang-wu FAN Guo-quan GAO Yan-ling ZHANG Wei ZHANG Shu Lü Wen-he Lü Dian-qiu 

作者机构：Northeast Agricultural University Harbin 150030 P.R. China Virus-free Seedling Research Institute Heilongjiang Academy of Agricultural Sciences Harbin 150086 PR China State Key Laboratory of Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2016年第15卷第2期

页      面：349-363页

核心收录：

学科分类：09[农学] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 0710[理学-生物学] 090603[农学-临床兽医学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the China Agriculture Research System(CARS-10-P14) the National Science Foundation for Post-doctoral Scientists of China(20110491125) the Heilongjiang Funds for Distinguished Young Scientist,China (JC 201018) the Harbin Application Technology Research and Development Projects,China(2013AE6AW059) the Young Scientists Science Foundation of Heilongjiang Province,China(QC2015026) 

主　　题：certification germplasm phylogenetic analysis quarantine seed potato viroid 

摘      要：China is the largest potato producing country worldwide,with this crop representing the fourth largest staple food crop in ***,the steady presence of Potato spindle tuber viroid（PSTVd） over the past five decades has a significant economic impact on potato *** determine why PSTVd control measures have been ineffective in China,more than 1 000 seed potatoes collected between 2009 and 2014 were subjected to PSTVd detection at the Supervision and Testing Center for Virus-free Seed Potatoes Quality,Ministry of Agriculture,China.A high PSTVd infection rate（6.5%） was detected among these commercial seed *** breeding lines of potato collected from 2012 to 2015 were also tested for PSTVd infection,revealing a high rate of PSTVd contamination in these potato propagation ***,comparison of the full-length sequences of 71 different Chinese PSTVd isolates revealed a total of 74 predominant PSTVd variants,which represented 42 different sequence variants of *** sequence analysis revealed 30 novel PSTVd sequence variants specific to *** phylogenetic analysis uncovered a close relationship between the Chinese PSTVd sequence variants and those isolated from *** is worth noting that three intermediate strains and six mild strains were identified among these *** results have important implications for explaining the ineffective control of PSTVd in China and thus could serve as a basic reference for designing more effective measures to eliminate PSTVd from China in the future.