A label-free colorimetric assay for detection of c-Myc mRNA based on peptide nucleic acid and silver nanoparticles

作     者：Xia Li Juan Song Bao-Li Chen Bing Wang Rui Li Hui-Min Jiang Ji-Feng Liu Chen-Zhong Li 

作者机构：Department of Chemistry Liaocheng UniversityLiaocheng 252059 China Key Laboratory of Food Nutrition and Safety Ministry ofEducation Tianjin University of Science and TechnologyTianjin 300457 China Nanobioengineering/Bioelectronics Laboratory Department ofBiomedical Engineering Florida International UniversityMiami FL 33174 USA 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2016年第61卷第4期

页      面：276-281页

核心收录：

学科分类：1002[医学-临床医学] 07[理学] 070302[理学-分析化学] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 

基　　金：the National Natural Science Foundation of China (21305058, 21205056, 21075058 and 21503104) Tai-Shan Scholar Research Fund of Shandong Province 

主　　题：c-myc mRNA 纳米银 比色法 无标记 肽核酸 检测 特异性识别 

摘      要：A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA/Ag NPs platform can undergo color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.