Restraint stress induces and exacerbates intestinal inflammation in interleukin-10 deficient mice

作     者：Seong-Joon Koh Ji Won Kim Byeong Gwan Kim Kook Lae Lee Joo Sung Kim 

作者机构：Department of Internal Medicine Seoul National University Boramae Hospital Seoul National University College of Medicine Department of Internal Medicine and Liver Research Institute Seoul National University College of Medicine 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2015年第21卷第28期

页      面：8580-8587页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by SNUH Research Fund,No.06-2011-1770 Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education,No.NRF-2014R1A1A2057695 

主　　题：Stress Colitis Interleukin-10 Inflammatorybowel disease Mouse model 

摘      要：AIM:To investigate the effects of restraint stress on chronic colitis in interleukin(IL)-10 deficient(IL-10^(-/-))***:The first experiment compared the effect of restraint stress on the development of intestinal inflammation in wild-type and IL-10^(-/-) *** wildtype and IL-10^(-/-) mice were physically restrained in a well-ventilated,50 cm3 conical polypropylene tube for2 h per day for three consecutive *** second experiment was performed to assess the effect of restraint stress on exacerbation of colitis induced by piroxicam in IL-10^(-/-) *** IL-10^(-/-) mice were exposed to restraint stress for 2 h per day for 3consecutive days,and then treated with piroxicam for4 d at a dose of 200 ppm administered in the rodent ***:In the first experiment,none of the wildtype mice with or without restraint stress showed clinical and histopathological abnormality in the ***,IL-10^(-/-) mice exposed to restraint stress exhibited histologically significant intestinal inflammation as compared to those without restraint *** the second experiment,restraint stress significantly reduced body weight and increased the severity of intestinal inflammation assessed by histopathologic grading in IL-10^(-/-) *** IL12p40 mRNA expression was strongly increased in mice exposed to restraint ***:This novel animal model could be useful in future study of psychological stress in the pathogenesis of inflammatory bowel disease.