真鲷天然抗性相关巨噬蛋白全长cDNA的克隆与序列分析

作     者：徐美瑜 陈松林 沙珍霞 季相山 XU Mei-Yu, CHEN Song-Lin, SHA Zhen-Xia, JI Xiang-Shan (Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Certificated by the Ministry of Agriculture, Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China)

作者机构：中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室 山东青岛　266071 

出 版 物：《水产学报》 (Journal of Fisheries of China)

年 卷 期：2005年第29卷第1期

页      面：128-132页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：国家863高技术研究发展项目(2002AA626010) 

主　　题：真鲷 天然抗性相关巨噬蛋白 cDNA末端快速扩增 序列分析 

摘      要：Natural resistance associated macrophage protein (Nramp) is an innate resistance protein to intracellular parasites, which is expressed plentifully in macrophage cells. Nramp has been studied in mouse, human, cattle, rainbow trout and channel catfish. However, little was known about the structure of Pagrus major Nramp. In order to get the complete sequence of Pagrus major Nramp, a pair of primer is designed according to a 200bp known sequence of Pagrus major Nramp cDNA. By the use of SMART RACE, the full Nramp of Pagrus major cDNA about 5 000 bp was obtained, including about 200 bp 5′ terminal region (UTR), complete encoding region and 3′ terminal region. There were 3 ployA signals, which showed many possibilities of cutting at 3′ terminal region. The character of Pagrus major Nramp nucleotide sequence and deduced amino acid sequence are analyzed. 12 putative transmembrane(TM) regions, a consensus transport motif (CTM), a predicted protein kinase C phosphroylation site and three predicted N-link glycosylation sites are indicated in its deduced amino acid sequence. The ’consense transport motif’ CTM is located between TM8 and TM9. Furthermore, a protein kinase C phosphroylation site and three N-link glycosylation sites were predicted. The alignment of amino acid sequences between Pagrus major Nramp cDNA and several animals is analyzed and the deduced amino acid sequence of Pagrus major Nramp had 77.8%, 83.0%, 82.3%, 80.0%, 81.1%, 60.4%, 70.3%, 58.5%, 69.5% identity with rainbow trout α(AAD20721), rainbow trout β (AAD20722), channel catfish(AF400108), fathead minnow (AAF01778), common carp (CAB60196), mouse 1(AAA39838), mouse 2(AAC42051), human 1(D50403), human 2(NP-000608), respectively. The alignment reveals high conservation in TM and CTM regions. Analysis result makes us get familiar with the structure and character of fish Nramp, furthermore, offers some information for the enhancement of immunity of fish and genetic amelioration on fish breeding.