Evaluation of antiviral activities of Houttuynia cordata ***,quercetin,quercetrin and cinanserin on murine coronavirus and dengue virus infection

作     者：K.H.Chiow M.C.Phoon Thomas Putti Benny K.H.Tan Vincent T.Chow 

作者机构：Department of MicrobiologyDepartment of Pathologyand Department of PharmacologyYong Loo Lin School of MedicineNational University Health SystemNational University of Singapore 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2016年第9卷第1期

页      面：1-7页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by a research grant from the National University of Singapore 

主　　题：Houttuynia cordata Murine coronavirus Dengue virus Quercetin Quercetrin Cinanserin 

摘      要：Objective:To evaluate the in vitro activities of the ethyl acetate(EA) fraction of Houttuynia cordata(***) Thunb.(Saururaceae) and three of its constituent flavonoids(*** and rutin) against murine coronavirus and dengue virus(DENV).Methods:The antiviral activities of various concentrations of the EA fraction of *** and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus(MHV) and DENV type 2(DENV-2).Cinanserin hydrochloride was also tested against *** EA fraction of *** was tested for acute oral toxicity in C57BL/6 ***:The EA fraction of *** inhibited viral infectivity up to 6 *** hydrochloride was able to inhibit MHV for only 2 *** 50%inhibitory concentrations(IC_(50)) of the EA fraction of *** added before the viral adsorption stage were 0.98 μg/mL for MHV and 7.50 μg/mL for DENV-2with absence of *** mice fed with the EA fraction up to 2 000 mg/kg did not induce any signs of acute toxicity,with normal histological features of major *** flavonoids exhibited comparatively weaker antiviral activity,notably quercetin which could inhibit both MHV and *** was followed by quercitrin which could inhibit DENV-2but not MHV,whereas rutin did not exert any inhibitory effect on either *** quercetin was combined with quercitrin,enhancement of anti-DENV-2 activity and reduced cytotoxicity were ***,the synergistic efficacy of the flavonoid combination was still less than that of the EA ***:The compounds in *** contribute to the superior antiviral efficacy of the EA fraction which lacked cytotoxicity in vitro and acute toxicity in *** has much potential for the development of antiviral agents against coronavirus and dengue infections.