Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase Cζ

作     者：Xiaoyan Zhang Huitu Liu Shengqing Ma 

作者机构：State Key Laboratory of Cell Proliferation and Regulation Biology Department of Biology Beijing Normal University Beijing China Department of Dermatology The First Hospital Beijing Medical University Beijing China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：1998年第43卷第18期

页      面：1556-1562页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：theNationalNaturalScienceFoundationofChina (GrantNos . 93880 0 2  392 0 0 0 2 4) 

主　　题：PKCζ human keratinocyte proliferation. 

摘      要：Protein kinase C (PKC), which has a wide variety of functions, comprises at least twelve isoenzymes that have distinct roles respectively. Research in recent years indicates that PKCζ plays a pivotal role in the regulation of cell proliferation. In order to investigate its role in the regulation of keratinocyte proliferation, eukaryotic expression plasmid of anti_sense PKCζ is constructed and transfected into human keratinocyte line, Colo16. The human keratinocyte model expressing anti_sense PKCζ is established successfully, the growth properties of the cell model is analyzed by the detection of cell growth curve and cell cycle phase. The mechanism of the relationship between PKCζ and CyclinD1 is also investigated by the western blotting analysis of CyclinD1 protein.