Possible Role of DNA Polymerase beta in Protecting Human Bronchial Epithelial Cells Against Cytotoxicity of Hydroquinone

作     者：DA-LIN HU HUAN-WEN TANG HAI-RONG LIANG DONG-SHENG TANG YI-MING LIU WEI-DONG JI JIAN-HUI YUAN YUN HE ZHENG-Yu ZHU JIAN-PING YANG DAO-KUI FANG YAN SHA XIAO-ZHI TU ZHI-XIONG ZHUANG 

作者机构：Department of Preventive Medicine School of Public Health Sun Yat-sen University Guangzhou 510080 Guangdong China Shenzhen Center for Disease Control and Prevention Shenzhen 518020 Guangdong China Medical College Foshan University Foshan 528000 Guangdong China School of Public HealthGuangdong Medical College Dongguan 523808 Guangdong China Department of Biochemistry and Molecular Biology Sun Yat-sen University Guangzhou 510080 Guangdong China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2007年第20卷第2期

页      面：171-177页

核心收录：

学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：This work was supported by a grant from the Major State Basic Research Development Program of China (No. 2002CB512904  2002CB512903) 

主　　题：Human bronchial epithelial cells RNA interference Hydroquinone Toxicology DNA polymerase beta 

摘      要：Objective To explore the toxicological mechanism of hydroquinone in human bronchial epithelial cells and to investigate whether DNA polymerase beta is involved in protecting cells from damage caused by hydroquinone. Methods DNA polymerase beta knock-down cell line was established via RNA interference as an experimental group. Normal human bronchial epithelial cells and cells transfected with the empty vector of pEGFP-C1 were used as controls. Cells were treated with different concentrations of hydroquinone （ranged from 10 μmol/L to 120 μmol/L） for 4 hours. MTT assay and Comet assay [single-cell gel electrophoresis （SCGE）] were performed respectively to detect the toxicity of hydroquinone. Results assay showed that DNA polymerase beta knock-down cells treated with different concentrations of hydroquinone had a lower absorbance value at 490 nm than the control cells in a dose-dependant manner. Comet assay revealed that different concentrations of hydroquinone caused more severe DNA damage in DNA polymerase beta knock-down cell line than in control cells and there was no significant difference in the two control groups. Conclusions Hydroquinone has significant toxicity to human bronchial epithelial cells and causes DNA damage. DNA polymerase beta knock-down cell line appears more sensitive to hydroquinone than the control cells. The results suggest that DNA polymerase beta is involved in protecting cells from damage caused by hydroquinone.