MRI of magnetically labeled mesenchymal stem cells in hepatic failure model

作     者：Kyu Ri Son Se Young Chung Hyo-Cheol Kim Hoe Suk Kim Seung Hong Choi Jeong Min Lee Woo Kyung Moon 

作者机构：Department of RadiologyKorea University Medical CenterAnam Hospital Department of RadiologySeoul National University College of MedicineInstitute of Radiation MedicineSeoul National University Medical Research Center Clinical Research InstituteSeoul National University Hospital 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2010年第16卷第44期

页      面：5611-5615页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Innovative Research Institute for Cell Therapy,No.A062260 Contract grant sponsor:National R&D Program for Cancer Control,Ministry of Health and Welfare,No.0920030 Basic Science Research Program Through the National Research Foundation funded by the Ministry of Education,Science and Technology,No.20090080219 Korea Healthcare Technology R&D Projects,Ministry for Health,Welfare and Family Affairs,Republic of Korea,No.A091001 

主　　题：Stem cell Liver failure Magnetic resonance Nanoparticle Transplantation 

摘      要：AIM:To track intravascularly transplanted mesenchymal stem cells (MSCs) labeled with superparamagnetic iron oxide (SPIO) by using magnetic resonance imaging (MRI) in an experimental rabbit model of hepatic ***:Human MSCs labeled with FDA-approved SPIO particles (Feridex) were transplanted via the mes-enteric vein into rabbits (n=16) with carbon tetrachloride-induced hepatic *** resonance (MR) examinations were performed with a 3.0 T clinical scanner immediately before and 2 h and 1,3,and 7 d after *** intensity (SI) changes on T2weighted MRI were measured,and correlation between MR findings and histomorphologic findings was also ***:SI on T2-weighted MRI decreased significantly in the liver 2 h after injection of human MSCs and returned gradually to the levels found before injection in 7 *** in SI in the liver at 2 h,1,3,and 7 d were 41.87% ± 9.63%,10.42% ± 4.3%,5.12% ± 1.9%,3.75% ± 1.2%,respectively (P 0.001).Histologic analyses confirmed the presence of MSCs in the liver,localized mainly in the sinusoids in early period (2 h and 1 d) and concentrated to the border zone in late period (3 and 7 d).The number of iron-positive cells in the liver at 2 h and on 1,3 and 7 d after transplantation was 29.2 ± 4.8,10.1 ± 3.7,6.7 ± 2.2,and 5.8 ± 2.1,respectively (P=0.013).CONCLUSION:Intravascularly injected SPIO-labeled MSCs in an experimental rabbit model of hepatic failure can be detected and followed with MRI.