Optimization of Enzymatic Extraction Process of Polysaccharides from Pseudostellaria heterophylla Fibrous Roots by Response Surface Methodology and Its Pilot Application

作     者：Huiqing PAN Qi ZHAO Yanda ZHANG Rongping YANG 

作者机构：Fujian Provincial Enterprise Technology Center of Fujian Beidi Pharmaceutical Co.Lid.Ningde 355300China Fujian Beidi Pharmaceutical Co.Ltd.Ningde 355300China Zherong County TaiZiShen AssociationNingde 355300China 

出 版 物：《Medicinal Plant》 (药用植物（英文）)

年 卷 期：2024年第15卷第5期

页      面：17-21页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Special Project of Central Leading Local Science and Technology Development(202113030) Regional Development Project of Fujian Provincial Science and Technology Plan(2022N3017) 

主　　题：Pseudostellaria heterophylla fibrous roots Polysaccharides Enzymatic extraction Pilot production Response surface opti timization 

摘      要：[Objectives]To study and optimize the process conditions of enzymatic hydrolysis technology for extracting polysaccharides from Pseudostellaria heterophylla fibrous roots and its application in workshop pilot tests.[Methods]*** fibrous roots were taken as the matrix material,and Box Behnken design was used to analyze the extraction time,composite enzyme addition amount,and liquid-solid ratio for response surface optimization experiments,and then applied to the pilot extraction of *** fibrous roots.[Results]Response surface analysis showed that all factors had a significant impact on the experimental *** optimal extraction process conditions for polysaccharides from *** fibrous roots were extraction time of 2.7 h,compound enzyme addition of 2.5%,and liquid-solid ratio of *** yield of polysaccharides from *** fibrous roots was 4.83%.The water extraction process of *** fibrous roots extraction pilot was used as the control group for response surface optimization of the pilot *** optimization results showed that the extraction time was 3 h,the amount of composite enzyme added was 2.5%,and the liquid-solid ratio was *** polysaccharide yield was 4.75%,an increase of 4.63%compared to the control group.[Conclusions]This paper could provide feasibility for the innovation of enzy-matic hydrolysis technology for *** fibrous roots and its workshop pilot practice application,as well as a reference for the industrial application of its medicinal resources.