p10 genes of Bombyx mori nuclear polyhedrosis virus and Autographa californica multiple nuclear polyhedrosis virus

作     者：张耀洲 吴祥甫 李载平 

作者机构：Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai 200031 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：1995年第38卷第1期

页      面：50-59页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Project supported by the 8th Five-Year Plan Research Program of China 

主　　题：Bombyx mori nuclear polyhedrosis virus Autogmpha californica multiple nuclear polyhedrosis virus nudeotide sequence of p10 p10 transfer vector CAT gene expression. 

摘      要：EcoR I-P fragment has been cloned from Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) genomic DNA and used as a probe. 0.5-kb and 1.1-kb fragments including p10 gene from Bombyx mori nuclear polyhedrosis virus (BmNPV) have been hybridized. The p10 ORF was located in the EcoR I-R fragment. Initiation codon ATG of p 10 from BmNPV has been mutated by PCR, and the ATG region became a Bgl II site. A novel transfer vector pBmAcPV-1 has been constructed using both the p10 5’-flanking region whose initiation codon ATG has been mutated with BmNPV and the p 10 3’-flanking region of AcMNPV. The vector can recombine with not only AcMNPV DNA to express foreign gene in Sf cells, but also BmNPV DNA to express foreign gene in Bm cells. CAT gene was expressed at high level in Bm cells under the control of the mutated p10 promoter of BmNPV.