Downregulation of MUC1 Inhibits Proliferation and Promotes Apoptosis by Inactivating NF-κB Signaling Pathway in Human Nasopharyngeal Carcinoma

作     者：WU Shou-Wu LIN Shao-Kun NIAN Zhong-Zhu WANG Xin-Wen LIN Wei-Nian ZHUANG Li-Ming WU Zhi-Sheng HUANG Zhi-Wei WANG A-Min GAO Ni-Li CHEN Jia-Wen YUAN Wen-Ting LU Kai-Xian LIAO Jun 吴首乌;林少坤;粘忠柱;王新文;林伟年;庄黎明;吴志圣;黄志伟;王阿敏;高妮莉;陈嘉雯;袁雯婷;卢凯娴;廖军

作者机构：Department of OtolaryngologyQuanzhou First HospitalQuanzhou 362001China Department of OtolaryngologyQuanzhou First Hospital Affiliated to Fujian Medical UniversityQuanzhou 362001China Department of UltrasoundQuanzhou First Hospital Affiliated to Fujian Medical UniversityQuanzhou 362001China Department of OrthopaedicsDongguan City People’s HospitalDongguan 523016China 

出 版 物：《生物化学与生物物理进展》 (Progress In Biochemistry and Biophysics)

年 卷 期：2024年第51卷第9期

页      面：2182-2193页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 100213[医学-耳鼻咽喉科学] 100214[医学-肿瘤学] 10[医学] 

基　　金：泉州市科技计划(2021N072S) 福建医科大学启航基金(2020QH1276)资助项目 

主　　题：mucin 1 nasopharyngeal carcinoma NF-κB signaling pathway proliferation apoptosis 

摘      要：Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory *** The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First *** expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with *** 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 *** qPCR and ELISA were executed to analyze the levels of TNF-αand *** blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of *** of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC *** Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.