Mining,characterization,and exploitation of EST-derived microsatellites in Gossypium barbadense

作     者：LU YuanDa CAI CaiPing WANG Lei LIN ShaoYan ZHAO Liang TIAN LiangLiang LU JunHong ZHANG TianZhen GUO WangZhen 

作者机构：National Key Laboratory of Crop Genetics & Germplasm Enhancement Cotton Research Institute Nanjing Agricultural University Nanjing210095 China 

出 版 物：《Chinese Science Bulletin》 (Chinese Science Bulletin)

年 卷 期：2010年第55卷第18期

页      面：1889-1893页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China (30730067 and 30871558) National High Technology Research and Development Program of China (2006AA10Z111) Natural Science Foun-dation of Jiangsu Province (BK2008036) Programme of Introducing Talents of Discipline to Universities (B08025) 

主　　题：美国东部 微卫星 挖掘 鉴定 海岛 开发 棉花 时间 

摘      要：Simple sequence repeats(SSRs) have been widely applied as molecular markers in genetic ***,the number of expressed sequence tags(ESTs) and SSR markers from Gossypium barbadense is fewer than those from other cotton *** this study,EST-SSR distribution from *** was characterized and new ***-derived EST-SSR markers were determined on the basis of the ESTs obtained by randomly sequencing 2 cDNA libraries associated with fiber development in *** mining 9697 non-redundant ESTs,a total of 638 SSR loci derived from 595 ESTs were *** ***,the frequency of ESTs containing SSRs was 6.13%,with an average of 1 SSR in every 10.4 kb of EST ***,trinucleotide was found to be the most abundant repeat type among 2-6-nucleotide repeat *** accounted for 26.6% of the total,followed by the hexanucleotide(26.0%) and pentanucleotide repeats(25.9%).Among all the repeat motifs,(AAG)n accounted for the highest ***-SSR primer pairs were developed using the Primer3 program,and the redundant primers were removed using the virtual PCR *** a result,380 non-redundant EST-SSR primer pairs were developed and used to detect polymorphisms between the mapping parents *** TM-1 and *** Hai7124 for constructing linkage groups in cultivated allotetraploid *** of these,98(25.8%) primer pairs detected ***,95 polymorphic loci from 82 primer pairs were integrated into the backbone genetic map;of these,42 were mapped into the A subgenome and 53 into the D *** present work provided the foundation for constructing saturated genetic maps and conducting comparative genomic studies on different cotton species.