Functionalized monodisperse microbubble production:microfluidic method for fast,controlled,and automated removal of excess coating material

作     者：M.R.P.van den Broek M.Versluis A.van den Berg T.Segers 

作者机构：BIOS/Lab on a Chip GroupMax Planck Center Twente for Complex Fluid DynamicsUniversity of TwenteEnschedeThe Netherlands Physics of Fluids GroupUniversity of TwenteEnschedeThe Netherlands 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2024年第10卷第4期

页      面：391-401页

核心收录：

学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

主　　题：bubble fluid method 

摘      要：Functionalized monodisperse microbubbles have the potential to boost the sensitivity and efficacy of molecular ultrasound imaging and targeted drug delivery using bubbles and *** bubbles can be produced in a microfluidic flow focusing ***,their functionalization and sequential use require removal of the excess lipids from the bubble suspension to minimize the use of expensive ligands and to avoid competitive binding and blocking of the receptor *** date,excess lipid removal is performed by centrifugation,which is labor intensive and challenging to *** importantly,as we show,the increased hydrostatic pressure during centrifugation can reduce bubble ***,we introduce a novel automated microfluidic’washing’***,bubbles are injected in a microfluidic chamber 1mm in height where they are left to float against the top ***,lipid-free medium is pumped through the chamber to remove excess lipids while the bubbles remain located at the top ***,the washed bubbles are resuspended and removed from the device into a collection *** demonstrate that the present method can(i)reduce the excess lipid concentration by 4 orders of magnitude,(ii)be fully automated,and(iii)be performed in minutes while the size distribution,functionality,and acoustic response of the bubbles remain ***,the presented method is a gateway to the fully automated production of functionalized monodisperse microbubbles.