Genetic Analysis and Linkage Mapping in a Resource Pig Population Using Microsatellite Markers

作     者：张敬虎 熊远 左波 雷明刚 蒋思文 李凤娥 郑嵘 李家连 Jinghu Zhang;Yuanzhu Xiong;Bo Zuo;Minggang Lei;Siwen Jiang;Feng'e Li;Rong Zheng;Jialian Li

作者机构：华中农业大学畜牧兽医学院农业部猪遗传与改良重点实验室 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2007年第34卷第1期

页      面：10-16页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 

基　　金：This work was supported by the National 973 Projects of China (No. 2006CB102102) and the National Natural Science Foundation of China (No. 30500358) 

主　　题：pig microsatellite marker linkage map heterozygosity 

摘      要：The use of markers and linkage map construction are important for QTL mapping in pigs. In this article, the genetic characteristics were studied and the linkage map was constructed in a pig resource population including 214 individuals by typing 39 microsatellite marker loci on Sus scrofa chromosomes, SSC4, SSC6, SSC7, SSC8, and SSC13. Results indicated that the average allele number, the average observed beterozygosity （Ho）, and the average polymorphism information content （PIC） in F1 and F2 population were 3.2, 0.528, 0.463 and 3.2, 0.496, 0.447, respectively. In the pig resource population, the average informative meiosis （IM） was 217.4 （44-316）, and the average linkage map length between the two sexes on the five chromosomes were 172.3 cM （SSC4）, 168.7 cM （SSC6）, 191.7 cM （SSC7）, 197.3 cM （SSC8）, and 178.3 cM （SSC13）. The orders of microsatellite marker loci in the linkage maps were identical to, but the length was greater than, those of USDA-MARC reference map. The results of this research showed the genetic relationship and genetic characteristics of the microsatellite markers in the pig resource family population, and the linkage map could be used to for QTL mapping in the subsequent study.