Cisplatin-loaded Polymer/Magnetite Composite Nanoparticles as Multifunctional Therapeutic Nanomedicine

作     者：Yan Zhang Xiao-ju Wang Miao Guo 阎虎生 Chen-hong Wang 刘克良 

作者机构：Key Laboratory of Functional Polymer Materials (Ministry of Education) and Institute of Polymer Chemistry Nankai University and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Beijing Institute of Pharmacology and Toxicology 

出 版 物：《Chinese Journal of Polymer Science》 (高分子科学（英文版）)

年 卷 期：2014年第32卷第10期

页      面：1329-1337页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 10[医学] 

基　　金：financially supported by the National Natural Science Foundation of China(Nos.51373080,81001417 and 20974052) PCSIRT(No.IRT1257) the National Key Technologies R&D Program for New Drugs of China(No.2009ZX09301-002) the Natural Science Foundation of Tianjin Municipality(No.09JCZDJC22900) 

主　　题：Drug delivery Folate Targeting Cisplatin Magnetic. 

摘      要：Multifunctional nanocarriers with multilayer core-shell architecture were prepared by coating superparamagnetic Fe3O4 nanoparticles with diblock copolymer folate-poly（ethylene glycol）-b-poly（glycerol monomethacrylate） （FA-PEG-b- PGMA）, and triblock copolymer methoxy poly（ethylene glycol）-b-poly（2-（dimethylamino） ethyl methacrylate）-b- poly（glycerol monomethacrylate） （MPEG-b-PDMA-b-PGMA）. The PGMA segment was attached to the surfaces of Fe304 nanoparticles, and the outer PEG shell imparted biocompatibility. In addition, folate was conjugated onto the surfaces of the nanocarriers. Cisplatin was then loaded into the nanocarrier by coordination between the Pt atom in cisplatin and the amine groups in the inner shell of the multilayer architecture. The loaded cisplatin showed pH-responsive release： slower release at pH 7.4 （i.e. mimicking the blood environment） and faster release at more acidic pH （i.e. mimicking endosome/lysosome conditions）. All of the cisplatin-loaded nanoparticles showed concentration-dependent cytotoxicity in HeLa cells. However, the folate-conjugated cisplatin-loaded carriers exhibited higher cytotoxicity in HeLa cells than non-folate conjugated cisplatin-loaded carriers.