Effect of Xinfeng capsule on pulmonary function in a adjuvant arthritis rat model

作     者：Lei Wan Jian Liu Chuanbing Huang Yuan Wang Li Zheng 

作者机构：Department of Rheumatism Immunitythe First Affiliated HospitalAnhui University of Chinese Medicine Department of Cancer BiologyCity of Hope National Medical Center and Beckman Research Institute 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2014年第34卷第1期

页      面：76-85页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation Project(No.81173211) Medical Key Subjects Chinese Paraly-sis Disease in the National School Construction Projects[Tra-ditional Chinese Medicine(2009)No.30] Anhui Science and Technology Office of Scientific Research Program(09020304046) Anhui Provincial Health Department of Tra-ditional Chinese Medicine Research Projects(No.2009-ZY05) Anhui Modern Chinese Medicine Basic and Applied Research and Development Projects Provincial Laboratory(2008 No.150) Anhui Medical and Technological Innovation Team project(No.2010TD005) 

主　　题：佐剂性关节炎 大鼠模型 肺功能 胶囊 新风 调节性T细胞 IFN-γ IL-4 

摘      要：OBjECTIVE:To observe the relationship between reduced pulmonary function and regulatory T cells(Tregs)and helper T cells(Th)1/Th2 drift in a rat model of adjuvant arthritis(AA),and to study the impact of Xinfeng capsule(XFC)on pulmonary function and investigate the mechanism of ***:Forty rats were randomly divided into normal control group(NC),model control group(MC),Tripterygium glycosides tablet group(TPT),and XFC group,with 10 in *** for the NCgroup,AA was induced in all rats by intracutaneous injection of 0.1 mL Freund s complete adjuvant in the right *** the 19th day after modeling,the NC and MC groups were given physiological saline(0.9%),while the TPT and XFC groups were given TPT(10 mg/kg)and XFC(2.4 g/kg),once daily,*** days after administration,changes in paw swelling,arthritis index(AI),pulmonary function,levels of serumγ-interferon(IFN-γ)and interleukin(IL)-4,Tregs in peripheral blood,and IFN-γ,IL-4,Forkhead box transcription factor 3(FoxP3)in lung tissue were observed by enzyme-linked immunosorbent assay,flow cytometry,polymerase chain reaction,and western ***:Compared with the NC group,paw swelling,AI,IFN-γ,and Th1/Th2 were increased,and pulmonary function parameters,IL-4,FoxP3 were decreased significantly in the MC group(P0.05 or P0.01).Pulmonary function parameters,Treg,IL-4,FoxP3(and mRNA)were higher,and paw swelling,AI,and IFN-γ(and mRNA)were lower in the XFC group than those in the MC *** XFC group was also much better than theTPT group in improving pulmonary function,FoxP3 mRNA,IFN-γ,IL-4,Th1/Th2,and IL-10(P0.05 or P0.01).CONCLUSION:Xinfeng capsule can improve pulmonary function by regulating the levels of Tregs,inhibiting the activation of Th1 to Th2 cells,inducing drift,maintaining cell immune suppression,correcting the imbalance of Th1/Th2,and reducing inflammatory mediators.