Investigation of structural change of purple membrane in storage by transmission electron microscope and atomic force microscope

作     者：LI Xiaodong LI Zongli YAN Jiusheng LI Hui HAN Baoshan HU Kunsheng WANG Aojin 

作者机构：Center of Condensed Matter PhysicsInstitute of PhysicsChinese Academy of SciencesBeijing 100080China State Key Laboratory of MagnetismInstitute of PhysicsChinese Academy of SciencesBeijing 100080China Photosynthesis Research CenterInstitute of BotanyChinese Academy of SciencesBeijing 100093China Institute of BiophysicsChinese Academy of SciencesBeijing 100101China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2001年第46卷第20期

页      面：1753-1756页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 07[理学] 

基　　金：This work was supported by the State Key Basic I Research and Development Plan Project (Grant No. 1998010102) 

主　　题：transmission electron microscope (TEM) atomic force microscope (AFM) purple membrane (PM) bacteriorhodopsin (BR). 

摘      要：The structural change of purple membrane during storage has been investigated by means of transmission electron microscope and atomic force microscope. It is found that many liposomes have spontaneously evolved from the purple membrane sheets isolated three years ago. The membrane proteins on the liposomes, bacteriorhodopsin, are still presented as trimers in 2-D hexagonal structure, which is the same as that in natural cell membrane. However, the cytoplasmic surface of purple membrane faced outside on the liposomes.