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Studies on the Temperature Effect on bacteriorhodopsin of Purple and Blue Membrane by Fluorescence and Absorption Spectroscopy

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 691-696页

作者： Lan-Ying CHENG~(1,2) Yue ZHANG~3 Shi-Gui LIU~1 Kun-Sheng HU~3 Kang-Cheng RUAN~2* 1 College of Life Science,Sichuan University,Chengdu 610064,China 2 State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Institute of Biophysics,Academia Sinica,Beijing 100101,China College of Life Science Sichuan UniversityChengdu 610064 China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Institute of Biophysics Academia SinicaBeijing 100101 China

Fluorescence and absorption spectra were used to study the temperature effect on theconformation of bacteriorhodopsin (bR) in the blue and purple membranes (termed as bRb and bRprespectively).The maximum emission wavelengths of tryptophan fluorescence in both proteins at roomtemperature are 340 nm,and the fluorescence quantum yield of bRb is about 1.4 fold higher than that of *** temperature increases,the tryptophan fluorescence of bRb decreases,while the tryptophan fluorescenceof bRp *** binding study of extrinsic fluorescent probe bis-ANS indicated that the probe can bindonly to bRb,but not to *** results suggest that significant structural difference existed between bRband *** was also found that both kinds of bR are highly thermal *** maximum wavelength of theprotein fluorescence emission only shifted from 340 nm to 346 nm at 100℃.More interestingly,as tempera-ture increased,the characteristic absorption peak of bRb at 605 nm decreased and a new absorption peak at380 nm *** transition occurred at a narrow temperature range (65℃-70℃).These facts indicatedthat an intermediate can be induced by high *** phenomenon has not been reported before.

关键词： bacteriorhodopsin purple membrane blue membrane fluorescence and absorption spectroscopy

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Tunable photocycle kinetics of a hybrid bacteriorhodopsin/quantum dot system

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Nano Research 2019年第2期12卷 365-373页

作者： Terianna J. Wax Jordan A. Greco Shutang Chen Nicole L. Wagner Jing Zhao Robert R. Birge 55 North Eagleville Rd Department of Chemistry University of Connecticut Storrs CT 06269-3060 USA

The inclusion of inorganic nanoparticles in biological environments has led to the creation of hybrid nanosystems that are employed in a variety of applications. One such system includes quantum dots (QDs) coupled with the photoactive protein, bacteriorhodopsin (BR), which has been explored in developing enhanced photovoltaic devices. In this work, we have discovered that the kinetics of the BR photocycle can be manipulated using CdSe/CdS (core/shell) QDs. The photocycle lifetime of protein samples with varying QD amounts were monitored using time-resolved absorption spectroscopy. Concentration-dependent elongations of the bR and M state lifetimes were observed in the kinetic traces, thus suggesting that excitonic coupling occurs between BR and QDs. We propose that the pairing of BR with QDs has the potential to be utilized in protein-based computing applications, specifically for real-time holographic processors, which depend on the temporal dynamics of the bR and M photointermediates.

关键词： bacteriorhodopsin photocycle bionanotechnology photochromism quantum dot hybrid materials

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Dynamics of Primary Events in the Photocycle of Excited bacteriorhodopsin

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 724-728页

作者： Jun-Jun LU, Ming MING, Yi YANG, Jia WU, Bo LI, Jian-Dong DING, Qing-Guo LI, and Shi-Xiong QIANDepartment of Physics, Fudan University, Shanghai 200433, China Department of Macromolecular Science, Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai 200433, China Department of Physics Fudan UniversityShanghai 200433 China Department of Macromolecular Science Key Laboratory of Molecular Engineering of Polymers Fudan UniversityShanghai 200433 China Department of Physiology and Biophysics School of Life Sciences Fudan UniversityShanghai 200433 China

Transient dynamic behavior of the excited bacteriorhodopsin (BR), which was isolated from the strain H. salinarum, was studied at excitation wavelength from 585 to 639 nm. With the one-color femtosecond (fs) pump-probe technique, we revealed the primary events in BR's photocycle that took place in an ultrafast time scale. From the analysis of the decay components of the dynamical traces, it was evident that the isomerization of the retinal

关键词： bacteriorhodopsin transient dynamics primary events femtosecond laser pump-probe technique

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Two orientations and photochromism of polyelectrolyte/bacteriorhodopsin alternative deposition multilayers

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Science China Chemistry 2000年第3期43卷 313-322页

作者：李美玲李宝芳江龙 Tapio Tussila Nikolai Tkachenko Helge Lemmetyinen Laboratory of Colloid and Interface Center of Molecular Science Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Institute of Materials Chemistry Tampere University of Technology FIN-33101 Tampere Finland

A series of organized multilayers have been formed by the alternative adsorption of positively charged poly(dimethyldiallylammonium chloride) (PDAC) and purple membrane (PM) fragments in suspensions at pH = 4—11. Both UV-vis spectrophotometry and quartz crystal micro-balance (QCM) technique were used to monitor the deposition process of PDAC/bacteriorhodopsin (bR) multilayers, suggesting that PM fragments and PDAC are deposited alternatively on the substrate uniformly. Upon illumination, all these multilayers generate photovoltages with defined signs. The negative sign of photovoltage accompanying the formation of M-state at pH <7 indicates that the extracellular side of PM fragments is directed toward the substrate; and the positive sign at pH≥7 indicates that the cytoplasmic side of PM fragments is directed toward the substrate. In addition, the long-lived multiple M-state has been observed in all multilayer films. Moreover, M-state at high pH, which shows the longer lifetime than that at low pH,

关键词： purple membrane bacteriorhodopsin photovoltage UV-vis adsorption spectra.

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Laser-induced bacteriorhodopsin LB films'fast photoelectric dynamics

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Science China Mathematics 1997年第7期40卷 761-766页

作者：姚保利徐大纶侯洵李宝芳江龙 State Key Laboratory of Transient Optics and Technology Xi'an Institute of Optics and Precision MechanicsChinese Academy of SciencesXi'an 710068China Institute of Photographic Chemistry Chinese Academy of SciencesBeijing 100101China

Ten layers of oriented bacteriorhodopsin (BR) LB films are prepared on ITO conductive glass toconstitute an ITO electrode/BR LB films/KCl solution/Cu electrode wet photocell.A Q-switched double frequency Nd:YAG laser with 10 Hz repetitive rate and 8 ns pulse width is used to excite the BR LB *** photoelectric signal is directly captured by a 300 MHz digitizing oscilloscope.A theory on the BR photoelectric dynamics is *** nonlinear least square fitting of the experimental curves acquired at different time bases,the experimental equation corresponding to the theoretical model is obtained,and the charge displacements and the decay time constants for each step in the BR photocycle are calculated.

关键词： bacteriorhodopsin LB film photoelectric dynamics.

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Electron microscopic observation and rotational diffusion measurement of bacteriorhodopsin in lipid vesicles

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Science China Chemistry 2001年第6期44卷 663-669页

作者：胡坤生王敖金 lanE.G.Morrison RichardJ.Cherry Institute of Biophysics Chinese Academy of Sciences Beijing China Department of Biological Science University of Essex UK

The morphology of bacteriorhodopsin reconstituted into dimyristoylphosphatidylcholine and egg-phosphatidylcholine vesicles was observed by freeze-fracture electron microscopy. The rotational diffusion of bacteriorhodopsin at different concentrations of melittin was measured by observing flash-induced transient dichroism in dimyristoylphosphatidylcholine vesicles. In the presence of melittin, bacteriorhodopsin molecules in dimyristoylphosphatidylcholine vesicles were aggregated into large particles or patches, and the ability of rotational diffusion of bacteriorhodopsin in vesicles was decreased. This suggests that melittin produces its effect via direct electrostatic interaction with bacteriorhodopsin. Low temperature-induced aggregation of bacteriorhodopsin was also observed in dimyristoylphosphatidylcholine vesicles. Low temperature may cause phase separation. bacteriorhodopsin was also successfully reconstituted into egg-phosphatidylcholine vesicles, but low temperature-induced aggregation of

关键词： bacteriorhodopsin Melittin 电子显微镜学旋转散开

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The　Responsivity　of　the　bacteriorhodopsin　Langmuir－Blodgett　Film　Intermittent　Light

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Chinese Chemical Letters 1996年第12期7卷 1131-1134页

作者： Wang, GY Yang, JH Zhang, ZG Lin, SH Lu, T Li, BF Jiang, L CAPITAL NORMAL UNIV INST BIOL INFORMAT SCIBEIJING 100037PEOPLES R CHINA ACAD SINICA INST PHOTOG CHEMBEIJING 100101PEOPLES R CHINA

ＴｈｅＲｅｓｐｏｎｓｉｖｉｔｙｏｆｔｈｅＢａｃｔｅｒｉｏｒｈｏｄｏｐｓｉｎＬａｎｇｍｕｉｒ－ＢｌｏｄｇｅｔｔＦｉｌｍＩｎｔｅｒｍｉｔｔｅｎｔＬｉｇｈｔ￥ＧｕａｎｇＹｕＷＡＮＧ；ＪｉａｎＨｕａＹＡＮＧ；ＺｈｉＧｕａｎｇ... 详细信息

ＴｈｅＲｅｓｐｏｎｓｉｖｉｔｙｏｆｔｈｅＢａｃｔｅｒｉｏｒｈｏｄｏｐｓｉｎＬａｎｇｍｕｉｒ－ＢｌｏｄｇｅｔｔＦｉｌｍＩｎｔｅｒｍｉｔｔｅｎｔＬｉｇｈｔ￥ＧｕａｎｇＹｕＷＡＮＧ；ＪｉａｎＨｕａＹＡＮＧ；ＺｈｉＧｕａｎｇＺＨＡＮＧ；ＳｈｕＨｕａｎｇＬＩＮ...

关键词： Responsivity the bacteriorhodopsin Blodgett

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Photocycle of 13-cis-retinal in bacteriorhodopsin caused by destruction to the cooperative effect of purple membrane

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Science China Chemistry 1998年第2期41卷 135-141页

作者：胡坤生何靳安张衡涛 Department of Cell Biology Institute of Biophysics Chinese Academy of Sciences Beijing China

The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X 100. The destruction to the cooperative effect of BR ultimately caused 13 cis retinal of the dark adapted BR to take part in BR photocycle and also to generate the deprotonated M 412 intermediate.

关键词： bacteriorhodopsin purple membrane cooperative effect Triton X 100 M 412 intermediate.

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Studies on HEPES Stabilizing M Intermediate of bacteriorhodopsin

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Chemical Research in Chinese Universities 1995年第3期11卷 215-218页

作者： Fu, Xue-qi Li, Zheng-qiang Wang, Jin-dui Wang, Bao-hui Li, Bo-fu Li, Tie-jin

EPES has proved to stabilize M intermediate of bacteriorhodopsin. Absorp-tion and Raman spectra showed that there was a more stable form of M intermedi-ate in BR-HEPES solution. It was found by SPS that there were two... 详细信息

关键词： bacteriorhodopsin M intermediate HEPES

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The 3rd-order nonlinearity of bacteriorhodopsin by four-wave mixing

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Chinese Science Bulletin 2000年第19期45卷 1752-1755页

作者： HUANG Yanping WANG Liying CHEN Shudi WANG Zugeng LI Qingguo Laboratory for Quantum Optics of East China Normal University Physics DepartmentEast China Normal UniversityShanghai 200062China Department of Physiology and Biophysics Fudan University.Shanghai 200433China

The 3rd-order nonlinear optical susceptibility x(3) and the response time of the light-transducing biomolecule bacteriorhodopsin were measured with the four-wave mixing technique and a picosecond frequency-doubled Nd: YAG laser (532 nm). The x(3) and the response time measured are 10-9 esu and 20 ps , respectively. The possible mechanism for generating the 3rd-order nonlinear optical susceptibility x(3) and response time were discussed.

关键词： bacteriorhodopsin 3rd-order nonlinearity four-wave mixing light-transducing biomolecule response time.

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