高效液相色谱法分离/蒸发光散射和紫外检测法测定天麻中天麻甙含量

作     者：魏泱 丁明玉 李红霞 WEI Yang;DING Ming-yu;LI Hong-Xia

作者机构：清华大学化学系北京100084 

出 版 物：《高等学校化学学报》 (Chemical Journal of Chinese Universities)

年 卷 期：2001年第22卷第4期

页      面：563-565页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：高效液相色谱法 蒸发光散射检测法 二极管阵列检测法 天麻 天麻甙 天然药物 含量测定 

摘      要：A normal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n hexane/methanol/ethyl acetate(volume ratio 6∶3∶2 ) as the mobile phase, was established for the direct determination of active constituents gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume . An silica gel column Zorbax RX SIL\[4 6 mm (\{i.d.\})×25 cm, 5 μm\] was employed. Regression equations revealed the linear relationships( r =0 998 and 0 999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104 22% and 97 65%, respectively. The contents of GA measured by ELSD and DAD are 13 9 mg/g(2 79% RSD) and 12 1 mg/g(2 99% RSD) respectively in Gastrodia elata Blume . The detection limits of ELSD and DAD( S/N =3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive.