Characterization of mycobacterium isolates from pulmomary tuberculosis suspected cases visiting Tuberculosis Reference Laboratory at Ethiopian Health and Nutrition Research Institute,Addis Ababa Ethiopia:a cross sectional study

作     者：Biniam Mathewos Nigatu Kebede Tesfu Kassa Adane Mihret Muluwork Getahun 

作者机构：Department of Immunology and Molecular biologySchool of Biomedical and Laboratory SciencesCollege of Medicine and Health SciencesUniversity of Gondar Aklilu Lemma Institute of PathobiologyCollege of Health SciencesAddis Ababa University Armaour Hanson Research Institute Ethiopian Health and Nutrition Research Institute 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2015年第8卷第1期

页      面：35-40页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：FIC NIH HHS [D43 TW009127] Funding Source: Medline 

主　　题：NTM Mycobacterium tuberculosis complex Mycobacteria growth indicator tube Lowenstein Jensen media 

摘      要：Objective:To characterize mycobaclerium isolates from pulmomary tuberculosis suspected cases visiting National Tuberculosis Reference Laboratory at Ethiopian Health and Nutrition Research Institute,for diagnosis of pulmonary tuberculosis from January 4 to February 22.2010 with total samples of ***:Sputum specimens were collected and processed:the deposits were *** culturing Lowenstein Jensen medium(LJ) and Mycobacteria Growth Indicator Tube(BACTEC MGIT 960) were *** Neo was used for detecting NTM isolates from isolates of BACTEC *** Armauer Hansen Research Institute,Addis Ababa Ethiopia,Deletion typing PCR method for species identification(from confirmed Mycobacterium tuberculosis complex(MTBC) isolates by Capilia Neo) uas ***:Out of 263 enrolled in the study.124 and 117 ol them were positive for mycobaeterium growth by BACTEC MGIT 960 and 1.1 culture method,*** BACTEC MGIT 960 positive media of 124 isolates.117 were randomly taken to perform Capilia TB Neo *** these 7(6%) of them were found to be NTM and 110(94%) were *** these 110 MTBC isolates,81 of them were randomly taken and run by the deletion typing RD9 PCR method of molecular *** of these 78(96.3%) were found to be species of Mycobacterium tuberculosis and 3(3.7%) were found to be not in the *** the types of methods of culture *** Growth Indicator Tube(BACTEC MGIT 960) method was found to have excellent agreement(with kappa value ol 0.78) with the routine method of ***:Pulmonary tuberculosis suspected cases visiting the National Tuberculosis Reference Laboratory at EHNR1 that were confirmed to be pulmonary tuberculosis are caused by the species of Mycobacterium *** treatment regimen including pyrazinamide can be applied to the patients as the first choice in the study area in Addis *** is indication of the presence of NTM in patients visiting the tuberculosis ref