Modulation effects of pressing manipulation on local inflammatory responses and ERK/NF-κB pathway in trigger point model rats

作     者：LIU Dan JIANG Quanrui KUANG Xiaoxia PAN Jieling ZENG Li LI Jiangshan LIU Xiaowei LI WU 刘丹;蒋全睿;匡小霞;潘杰灵;曾理;李江山;刘小卫;李武

作者机构：Hunan University of Chinese MedicineChangsha 410208China Guangxi University of Chinese MedicineNanning 530001China The Second HospitalUniversity of South ChinaHengyang 421000China 

出 版 物：《Journal of Acupuncture and Tuina Science》 (针灸推拿医学（英文版）)

年 卷 期：2024年第22卷第5期

页      面：371-380页

核心收录：

学科分类：1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：国家自然科学基金项目,No.82174526,No.82274676,No.82374613 湖南省自然科学基金项目,No.2021JJ40482,No.2023JJ30458 湖南省科技创新计划,No.2022RC1221 

主　　题：Tuina Massage Pressing Manipulation Ashi Point Trigger Points ERK/NF-κB Signaling Pathway Inflammation Rats 

摘      要：Objective:To investigate the mechanism of trigger point deactivation induced by pressing manipulation in a rat model and to explore its potential regulation of the inflammatory response through the extracellular signal-regulated kinase(ERK)/nuclear factor-κB(NF-κB)***:Fifty male Sprague-Dawley rats were randomly divided into a blank group,a model group,a pressing manipulation group,an ERK agonist group,and a pressing manipulation+ERK agonist group,with 10 rats in each *** for the blank group,rats in other groups were used to establish the trigger point rat model using the blunt blow combined with the eccentric exercise *** pressing manipulation group underwent pressing manipulation intervention at the trigger *** ERK agonist group received an injection of recombinant human epidermal growth factor via the tail *** pressing manipulation+ERK agonist group received interventions from both the pressing manipulation and ERK agonist *** pressure pain threshold(PPT)was measured by a mechanical pain threshold detector before and after the *** histological changes were evaluated by hematoxylin-eosin staining after the intervention;the expression levels of ERK,phosphorylated ERK(p-ERK),NF-κB p65(p65),phosphorylated NF-κB p65(p-p65),and phosphorylated NF-κB inhibitor(p-IκB)were detected by Western blotting;the levels of interleukin(IL)-1β,IL-6,and tumor necrosis factor(TNF)-αwere detected by enzyme-linked immunosorbent ***:The PPT increased(P0.05);the inflammatory cells disappeared;the ratios of p-ERK/ERK,p-p65/p65,and p-IκB/β-actin,also the levels of IL-1β,IL-6,and TNF-αall decreased in the pressing manipulation group after the intervention compared with the model group(P0.05).The PPT decreased significantly(P0.05),the inflammatory cell presence increased,and the ratios of p-ERK/ERK and p-p65/p65 were elevated(P0.05);additionally,the levels of IL-6 and TNF-αwere significantly higher in the pressing manip