Electrospinning/3D printing-integrated porous scaffold guides oral tissue regeneration in beagles

作     者：Li Yuan Chen Yuan Jiawei Wei Shue Jin Yi Zuo Yubao Li Xinjie Liang Jidong Li Li Yuan;Chen Yuan;Jiawei Wei;Shue Jin;Yi Zuo;Yubao Li;Xinjie Liang;Jidong Li

作者机构：Research Center for Nano-BiomaterialsAnalytical and Testing CenterSichuan UniversityChengdu 610064China ZXTGY(Jiangsu)Technology Co.Ltd.Suzhou 215400China 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2024年第7卷第6期

页      面：1000-1017页

核心收录：

学科分类：08[工学] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：supported by the National Natural Science Foundation of China(No.32371398) the Sichuan International Science and Technology Innovation Cooperation Project,China(No.2023YFH0064) the National Key Research and Development Program of China(No.2016YFA0201700) the Fundamental Research Funds for the Central Universities,China 

主　　题：Electrospinning 3D printing Gradient porous scaffold Oral tissue regeneration 

摘      要：The combined use of guided tissue/bone regeneration(GTR/GBR)membranes and bone filling grafts represents a classical therapy for guiding the regeneration and functional reconstruction of oral soft and hard ***,due to its displacement and poor mechanical support,bone meal is not suitable for implantation in the case of insufficient cortical bone support and large dimensional *** combination of GTR/GBR membrane with a three-dimensional(3D)porous scaffold may offer a resolution for the repair and functional reconstruction of large soft and hard tissue *** this study,a novel integrated gradient biodegradable porous scaffold was prepared by bonding a poly(lactic-co-glycolic acid)(PLGA)/fish collagen(FC)electrospun membrane(PFC)to a 3D-printed PLGA/nano-hydroxyapatite(HA)(PHA)*** consistency of the composition(PLGA)ensured strong interfacial bonding between the upper fibrous membrane and the lower 3D *** vitro cell experiments showed that the PFC membrane(upper layer)effectively prevented the unwanted migration of L929 *** in vivo investigations with an oral soft and hard tissue defect model in beagles revealed that the integrated scaffold effectively guided the regeneration of defective oral *** results suggest that the designed integrated scaffold has great potential for guiding the regeneration and reconstruction of large oral soft and hard tissues.