Signal interference between drugs and metabolites in LC-ESI-MS quantitative analysis and its evaluation strategy
作者机构：Institute of Clinical PharmacologySchool of Pharmaceutical SciencesSun Yat-sen UniversityGuangzhou510080China Guangdong RangerBio Technologies Co.Ltd.DongguanGuangdong523000China
出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)
年 卷 期：2024年第14卷第7期
页 面：1024-1034页
核心收录：
学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学]
基 金：support provided by the National Natural Science Foundation of China(Grant No.:82173776) Natural Science Foundation of Guangdong Province,China(Grant No.:2021A1515010574) Guangdong Basic and Applied Basic Research Foundation,China(Grant No.:2021A1515110346)
主 题：Liquid chromatography-electrospray ionization tandem mass spectrometry(LCESI-MS) Drugs Metabolites Ionization interference Quantitative analysis
摘 要：Liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS)is a widely utilized technique for in vivo pharmaceutical *** interference within electrospray ion source,occurring between drugs and metabolites,can lead to signal variations,potentially compromising quantitative ***,method validation often overlooks this type of signal interference,which may result in systematic errors in quantitative results without matrix-matched *** this study,we conducted an investigation using ten different groups of drugs and their corresponding metabolites across three LC-ESI-MS systems to assess the prevalence of signal *** interferences can potentially cause or enhance nonlinearity in the calibration curves of drugs and metabolites,thereby altering the relationship between analyte response and concentration for ***,we established an evaluation scheme through a step-by-step dilution assay and employed three resolution methods:chromatographic separation,dilution,and stable labeled isotope internal standards *** above strategies were integrated into the method establishment process to improve quantitative accuracy.
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