Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp.(Phaeophyta,Sargassaceae) collected from Gulf of Mannar

作     者：Kajal Chakraborty Nammunayathuputhenkotta Krishnankartha Praveen Kodayan Kizekadath Vijayan Gonugontla Syda Rao 

作者机构：Marine Biotechnology DivisionCentral Marine Fisheries Research Institute Central Marine Fisheries Research Institute 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2013年第3卷第1期

页      面：8-16页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the funding under the Science and Engineering Research Council(SERC)Scheme(SR/FTP/CS-63/2007)from Department of Science and Technology.New Delhi.India 

主　　题：Brown seaweeds Turbinaria conoides Turbinaria ornata Antioxidant activity Total phenolic contents 

摘      要：Objective:To evaluate the antioxidant activities and total phenolic contents of brown seaweeds belonging to Turbinaria spp.[Turbinaria conoides(***)and Turbinaria ornata(***)collected from Gulf of Mannar of southeastern coast of India in various in vitro ***:The antioxidant activity was evaluated using different in vitro systems,viz.,I,I-diphenyl-2-picrylhydrazyl(DPPH),2,2 -azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium salt(ABTS),H_20_2/H0 radical scavenging,Fe^(2+)ion chelating ability,and reducing ***-Ciocalteu method was used to determine the total phenolic content of the extracts,and the results were expressed as mg of gallic acid equivalents(GE)/g of the seaweed *** acid-reactive substances assay was employed to assess the ability of the seaweed extracts to inhibit lipid ***:Ethyl acetate(EtOAc)fraction of *** registered significantly higher phenolic content(105.97 mg GE/g)than that of ***(69.63 mg GE/g).Significantly higher antioxidant potential as determined by DPPH(64.14%)radical scavenging activity was registered in EtOAc fraction of ***.A higher ABTS(?)radical scavenging(IC_(50)3.16μg/mL),Fe^(2+)chelating(IC_(50)0.46 mg/mL),H_2O_2 scavenging(IC_(50)4.25 mg/mL),lipid peroxidation inhibitory(TBARS,IC_(50)0.21 mg/mL),and reducing abilities(IC_(50)52.67 mg/mL)(P0.05)were realized in EtOAc fraction of *** than other ***:This study indicated the potential use of *** and *** as candidate species to be used as food supplements/functional foods to increase shelf-life of food items for human consumption,and nutraceuticals to deter deleterious free radical-induced life-threatening diseases.