Influence of 6-BA on Incidence Rate of Chinese Cabbage Clubroot (Plasmodiophora brassicae Woron)

作     者：王哲 郑萍 刘照斌 马有会 王火旭 

作者机构：辽宁师范大学生命科学学院辽宁大连116081 临西县技工学校河北邢台054900 辽宁师范大学分析测试中心辽宁大连116029 辽宁师范大学生命科学学院辽宁省生物技术重点实验室辽宁大连116081 

出 版 物：《Plant Diseases and Pests》 (植物病虫害研究（英文版）)

年 卷 期：2011年第2卷第5期

页      面：21-24页

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

主　　题：6-BA Chinese cabbage Clubroot Scanning electronic microscope PCR technology China 

摘      要：[Objective] The paper was to study the relationship between cytokinin and Chinese cabbage clubroot, and to explore the incidence mechanism of clubroot. [Method]The spores were extracted from the tumors of diseased plant, and their DNA was extracted for PCR test. The diagnosed spores were inoculated to the culture soils in erlenmeyer flask at seeding stage, at seed germination and 21 d after sowing, and 0.08 μmol/L 6-BA was added to the soil at germination. Spores were extracted from the root tumors developed in inoculated plants and examined by scanning electronic microscope （ SEM）, and the incidence rate of clubroot in 6-BA treatment and control was recorded. [ Result] Plasmodiophora brassicae Woron could cause the clubroot incidence of Chinese cabbage planted in erlenmeyer flasks, the incidence rate of chibroot in the treatment adding with 0.08 μmol/L 6-BA was 100%, while that in the treatment without 6-BA was 57%, and the volume of former tumor was much larger than the latter. SEM showed that the size of resting spore of P. brassicae was 1.5 -4.3μm. [ Conduslon] 6-BA applied at germination could significantly promote the formation of tumor of Chinese cabbage clubroot.