A simple and versatile protocol for isolation of RNA from plant, fungi and animal

作     者：QUE You-xiong LI Wei XU Jing-sheng XU Li-ping ZHANG Mu-qing 

作者机构：Key Lab of Sugarcane Eco-Physiology ＆ Genetic Improvement Ministo of Agriculture Fuzhou 350002 China 

出 版 物：《Journal of Agricultural Science and Technology》 (农业科学与技术（美国大卫英文）)

年 卷 期：2008年第2卷第1期

页      面：63-65页

学科分类：0710[理学-生物学] 12[管理学] 071010[理学-生物化学与分子生物学] 1201[管理学-管理科学与工程(可授管理学、工学学位)] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 081201[工学-计算机系统结构] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：This research was financially supported by the National Natural Science Foundation of China (30170639) and the China High Technology (863) Project (2007AA100701) 

主　　题：Saccharum officinarum RNA polysaccharides polyphenols 

摘      要：Compared to other plants, the traditional protocols for RNA isolation from sugarcane （Saccharum） are usually cJeficient and result in poor yields, or low-quality. Here we describe a modified method for isolating RNA from various fissoes of S. officinarum, in which only Trizol and some routine reagents were needed. The absorbance at 260/280 nm ranges from 1.70 to 1.80, indicating that the quality of RNA is high, as confirmed by double-stranded cDNA synthesis and reverse ~ranscription-polymerase chain （RT-PCR）. This protocol can also be used effectively in RNA isolation from Erianthus arundinaceus, rice （Oryza sativa Linnaeus）, Achyranthes, sporotrichum spp., Thermophil and loach （Misgurnus an g uillicaudatus ）.