Synthesis and characterization of a pH-sensitive shielding system for polycation gene carriers

作     者：XIA JiaLiang1,2,CHEN Jie1,TIAN HuaYu1 & CHEN XueSi1 1Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China 2Graduate University of Chinese Academy of Sciences,Beijing 100049,China 

作者机构：Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun China Graduate University of Chinese Academy of Sciences Beijing China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2010年第53卷第3期

页      面：502-507页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (Grant Nos 20604028,50873102,50733003,A3 Foresight Program 20621140369) Ministry of Science and Technology of China (Grant No 2007DFR5020) 

主　　题：gene delivery shielding system pH-sensitive polyglutamate 

摘      要：To increase the in vivo stability of polycation gene carriers,a pH-sensitive shielding system,γ-benzyl L-glutamate-co-glutamate acid polymer(PGA(60)(60 refers to the molar ratio of glutamate acid in the polymer)),was synthesized and ***(60) showed pH sensitivity at about pH ***(60) shielded the positive charge of DNA/PEI(1:1) *** retardation assay showed that no DNA-strand exchange with PGA(60) occurred after PGA(60) was added to DNA/PEI complexes at different *** cytotoxicity tests demonstrated that neither PGA(60) nor DNA/PEI/PGA(60) ternary complexes had cytotoxicity at the test *** transfection efficiency was improved when the positive charge was partly shielded by PGA(60).Because of the charge repulsion between the surface of cells and ternary complex particles,there was almost no transfection efficiency when the zeta potential of ternary complexes turned to *** of the suitable pH sensitive range,PGA(60) may be a potential shielding system for polycation gene carriers to be used in vivo.