Massively parallel characterization of regulatory elements in the developing human cortex
作者机构：不详
出 版 物：《四川生理科学杂志》 (Sichuan Journal of Physiological Sciences)
年 卷 期：2024年第46卷第5期
页 面：1085-1085页
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
摘 要：Nucleotide changes in gene regulatory elements are important determinants of neuronal development and *** massively parallel reporter assays in primary human cells from mid-gestation cortex and cerebral organoids,we interrogated the cis-regulatory activity of 102,767 open chromatin regions,including thousands of sequences with cell type-specific accessibility and variants associated with brain gene *** primary cells,we identified 46,802 active enhancer sequences and 164 variants that alter enhancer *** was comparable in organoids and primary cells,suggesting that organoids provide an adequate model for the developing *** deep learning we decoded the sequence basis and upstream regulators of enhancer *** work establishes a comprehensive catalog of functional gene regulatory elements and variants in human neuronal development.
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