Acoustofluidic scanning fluorescence nanoscopy with a large field of view
作者机构：Thomas Lord Department of Mechanical Engineering and Materials ScienceDuke UniversityDurhamNC 27708USA Department of Biomedical EngineeringDuke UniversityDurhamNC 27708USA The MITRE CorporationMcLeanVA 22102USA
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2024年第10卷第3期
页 面：159-167页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：support from the National Institutes of Health(R01GM143439,R01HD103727,UH3TR002978,and U18TR003778) the National Science Foundation(CMMI-2104295) a National Science Foundation Graduate Research Fellowship under Grant no.2139754
主 题：field resolution utilize
摘 要：Large-field nanoscale fluorescence imaging is invaluable for many applications,such as imaging subcellular structures,visualizing protein interactions,and high-resolution tissue ***,conventional fluorescence microscopy requires a trade-off between resolution and field of view due to the nature of the optics used to form the *** overcome this barrier,we developed an acoustofluidic scanning fluorescence nanoscope that simultaneously achieves superior resolution,a large field of view,and strong fluorescent *** acoustofluidic scanning fluorescence nanoscope utilizes the superresolution capabilities of microspheres that are controlled by a programmable acoustofluidic device for rapid fluorescence enhancement and *** acoustofluidic scanning fluorescence nanoscope resolves structures that cannot be resolved with conventional fluorescence microscopes with the same objective lens and enhances the fluorescent signal by a factor of~5 without altering the field of view of the *** improved resolution realized with enhanced fluorescent signals and the large field of view achieved via acoustofluidic scanning fluorescence nanoscopy provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine,biology,biophysics,and biomedical engineering.
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