Revolutionizing Antimicrobial Biomaterials:Integrating an Enzyme Degradation‑Resistant Sequence into Self‑Assembled Nanosystems to Overcome Stability Limitations of Peptide‑Based Drugs

作     者：Weikang Yu Xu Guo Qingrui Li Xuefeng Li Yingxin Wei Changxuan Shao Licong Zhang Jiajun Wang Anshan Shan 

作者机构：College of Animal Science and TechnologyNortheast Agricultural UniversityHarbin 150030People’s Republic of China 

出 版 物：《Advanced Fiber Materials》 (先进纤维材料（英文）)

年 卷 期：2024年第6卷第4期

页      面：1188-1211页

核心收录：

学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.32030101,32272914,and 32002215) the Heilongjiang Provincial Collaborative Innovation Achievement Project for Universities—Incubation Project(LJGXCG2022-022) the Heilongjiang Touyan Innovation Team Program(No.54600112) the Natural Science Foundation of Heilongjiang Province(YQ2022C015) 

主　　题：Naphthyl Nanofibers Antimicrobial peptide Self-assembled Protease resistance Antibacterial mechanism 

摘      要：Incorporating enzyme-resistant peptide sequences into self-assembled nanosystems is a promising strategy to enhance the stability and versatility of peptide-based antibacterial drugs,aiming to replace ineffective *** combining newly designed enzymatic-resistant sequences with synthetically derived compounds bearing single,double,triple,or quadruple aromatic rings.A series of nanoscale antimicrobial self-assembled short peptides for the purpose of combating bacterial infections are ***^(*)(Nap–DNal–Nal–Dab–Dab–NH2,where Nap represents the 1-naphthylacetyl group)possesses the greatest clinical potential(GMSI=23.96)among the peptides in this *** high concentrations in an aqueous environment,Nap^(*)spontaneously generates nanofibers to capture bacteria and prevent their evasion,exhibiting broad-spectrum antimicrobial effects and exceptional *** the presence of physiological salt ions and serum,the antimicrobial agent exhibits strong effectiveness and retains impressive resistance even when exposed to high levels of proteases(trypsin,chymotrypsin,pepsin).Nap^(*)exhibits negligible in vivo toxicity and effectively alleviates systemic bacterial infections in ***,Nap^(*)initially captures bacteria and induces bacterial cell death primarily through membrane dissolution,achieved by multiple synergistic *** summary,these advances have the potential to greatly expedite the clinical evolution of nanomaterials based on short peptides combined with naphthyl groups and foster the development of peptides integrated with self-assembled systems in this domain.