In vitro and in vivo evaluation of effects of Mg-6Zn alloy on tight junction of intestinal epithelial cell

作     者：于嵩 王啸虎 陈义刚 郑起 张小农 赵常利 张绍翔 阎钧 Song YU;Xiao-hu WANG;Yi-gang CHEN;Qi ZHENG;Xiao-nong ZHANG;Chang-li ZHAO;Shao-xiang ZHANG;Jun YAN

作者机构：上海交通大学附属第六人民医院普通外科上海200233 上海交通大学医学院上海200025 南京医科大学附属无锡市第二人民医院普通外科南京214002 上海交通大学材料学院金属基复合材料国家重点实验室上海200240 苏州奥芮济医疗科技有限公司苏州215513 

出 版 物：《Transactions of Nonferrous Metals Society of China》 (中国有色金属学报（英文版）)

年 卷 期：2015年第25卷第11期

页      面：3760-3766页

核心收录：

学科分类：08[工学] 1002[医学-临床医学] 0806[工学-冶金工程] 0818[工学-地质资源与地质工程] 0815[工学-水利工程] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0813[工学-建筑学] 0703[理学-化学] 0814[工学-土木工程] 0702[理学-物理学] 10[医学] 

基　　金：Project(30901422)supported by the National Natural Science Foundation of China Project(YG2010MS45)supported by Shanghai Jiao Tong University Interdisciplinary(Biomedical Engineering)Research Fund,China Project(09XJ21005)supported by School of Medicine Science and Technology Fund,Shanghai Jiao Tong University,China 

主　　题：Mg-6Zn alloy intestinal epithelial cell tight junction 

摘      要：The effects of biodegradable Mg?6Zn alloy on tight junction of intestinal epithelial cells (IEC-6) were investigated. In the in vitro experiments, the cells were exposed to Mg?6Zn alloy extracts with different concentrations (0, 20% and 40%) for 1, 3 and 5 d. The real-time polymerase chain reaction (PCR) results show that when the cells are treated with 40% and 20% extracts, the expression of Zona Occludens 1 (ZO-1) and Occludin increase as compared with those in the control group. In the in vivo experiments, Mg?6Zn alloy and titanium staples were implanted into rabbits’ intestinal tract for 1, 2 and 3 weeks. By immunohistochemical staining of peri-implant intestinal tissue, increased expression of Occludin and ZO-1 are observed in the Mg?6Zn alloy groups as compared with those in the titanium and control groups. The results show that Mg?6Zn alloy in intestine may promote the regeneration of tight junction, and the extract with a certain concentration can induce the expression of tight junction related genes in IEC-6 cells.