Upregulation of REL and WSB1 in Patients With Psoriasis and Metabolic Syndrome

作     者：Manahel Mahmood Alsabbagh Moiz Bakhiet Safa Taha Manahel Mahmood Alsabbagh;Moiz Bakhiet;Safa Taha

作者机构：Princess Al-Jawhara Center for Molecular Medicine and Inherited DisordersDepartment of Molecular MedicineArabian Gulf UniversityManama 329Kingdom of Bahrain. 

出 版 物：《International Journal of Dermatology and Venereology》 (国际皮肤性病学杂志（英文）)

年 卷 期：2023年第6卷第4期

页      面：189-194页

核心收录：

学科分类：1002[医学-临床医学] 10[医学] 100206[医学-皮肤病与性病学] 

基　　金：supported by Arabian Gulf University  Bahrain(No. E005-PI-04/17)。 

主　　题：psoriasis diabetes mellitus hypertension inflammation metabolic syndrome obesity REL WSB1 

摘      要：Objective:A strong association exists between metabolic syndrome and psoriasis.The current study was performed to explore the gene regulation of metabolic syndrome in patients with psoriasis.Methods:Patients were assessed for psoriasis and metabolic syndrome clinically (Psoriasis Area and Severity Index,height,weight,waist circumference,and blood pressure) and biochemically (lipid profile and fasting blood sugar concentration).Systemic gene regulation was first explored by microarray and analyzed using Transcriptome Analysis Console Software,after which two selected upregulated genes were further validated using polymerase chain reaction and enzyme-linked immunosorbent assay and analyzed using independent samplet test.Results:The analysis showed 7,269 upregulated and 3 downregulated genes at a fold change of 2 andP value of 0.05;only 17 genes were upregulated and none were downregulated at a fold change of 8 andP value of 0.005.Comparison with 22 previously reported potential biomarkers of metabolic syndrome in patients with psoriasis showed that the levels of 16 biomarkers aligned with the gene regulation observed in the current study.In particular,theREL transcript was upregulated 12-fold (P = 8.16 × 10-17),while theWSB1 transcript was upregulated 9-fold (P = 9.87 × 10-13).Validation showed thatREL was also upregulated 2-fold in the polymerase chain reaction,and its protein was expressed at 7.140 ng/mLvs.undetectable levels in the cases (P = 0.048).However,WSB1 was upregulated 2-fold in the polymerase chain reaction compared with controls,and unexpectedly,its protein was undetectable in cases but detectable in controls (P = 0.018).Conclusion:The upregulation ofREL andWSB1 was observed in patients with psoriasis and metabolic syndrome,the clinical application ofREL andWSB1 as biomarkers needs further validation for potential future implications in clinical practice.