Engineer and split an efficient hypercompact CRISPR–CasΦ genome editor in plants

作     者：Yan Sun Jianjian Hu Zhichao Hu Hejie Zhou Yuhong Gao Yini Liu Yuan Ji Gencheng Xu Yifan Guo Yuanyan Zhang Yunlu Tian Xi Liu Shirong Zhou Yuqiang Liu Tingdong Li Chao Li Jianmin Wan 

作者机构：State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationJiangsu Engineering Research Center for Plant Genome EditingNational Observation and Research Station of Rice Germplasm ResourcesNanjing Agricultural UniversityNanjing 210095China Zhongshan Biological Breeding LaboratoryNanjing 210095China Sanya Institute of Nanjing Agricultural UniversityHainan Seed Industry LaboratorySanya 572025China State Key Laboratory of Crop Stress Biology for Arid AreasCollege of AgronomyNorthwest A&F UniversityYangling 712100China State Key Laboratory of Crop Gene Resources and BreedingInstitute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China These authors contributed equally to this article 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2024年第5卷第7期

页      面：8-11页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the Ministry of Agriculture and Rural Affairs of China,the Jiangsu Province Natural Science Foundation(grant nos.BK20212010 and BK20230038) the Jiangsu Province Key Research and Development Program(grant no.BE2022383) the Hainan Seed Industry Laboratory(grant no.B21HJ1004) the Zhongshan Biological Breeding Laboratory(grant no.ZSBBL-KY2023-04) the Guidance Foundation of the Sanya Institute of Nanjing Agricultural University(grant no.NAUSY-ZZ01) the Bingtuan Key Science and Technology Program of Xinjiang Province(grant no.2023AB006-02) the National Natural Science Foundation of China(grant no.31872806) 

主　　题：CRISPR genome editor 

摘      要：The programmable CRISPR-Cas genome editing technology,adopted from prokaryotic adaptive immune systems,has revolutionized genome engineering in plants(Liu et al.,2022a).Many efforts have been made to improve the activity,specificity,and protospacer adjacent motif(PAM)variants of Class 2 Cas nucleases,such as Cas9,Cas12a,and Cas12b(Liu et al.,2022a).However,their large size(∼1000–1400 amino acids)poses a challenge in scenarios requiring a compact Cas nuclease,particularly in urgent situations like plant virus-induced genome editing(Cheuk and Houde,2018;Li et al.,2021;Varanda et al.,2021).