Protein expression changes in Tibetan middle-to-long distance runners after the transition from high altitude to low altitude:Implications for enhancing endurance training

作     者：Di Wang Weiping Shu 

作者机构：Chengdu Sport UniversityChengduSichuanChina 

出 版 物：《Sports Medicine and Health Science》 (运动医学与健康科学（英文）)

年 卷 期：2024年第6卷第4期

页      面：370-377页

学科分类：070801[理学-固体地球物理学] 07[理学] 0708[理学-地球物理学] 

主　　题：Proteomic analysis Tibetan Middle-to-long distance runner Alternate training CFTR CAI 

摘      要：The study aims to investigate the differences in protein expressions in Xizang s(Tibetan)middle-to-long distance runners after the transition from high altitude to low altitude and reveal the molecular mechanisms underlying their enhanced middle-to-long distance running *** the study,eleven subjects were selected from native Tibetan middle-to-long distance runners to participate in an 8-week pre-competition exercise training program consisting of a 6-week training stage in Kangding City at an altitude of 2560 meters(m)and a subsequent 2-week training stage in Leshan City at an altitude of 360 *** samples were collected twice from the runners before beginning altitude exercise training in Kangding and after going to sea level-Leshan *** a label-free quantitative method,peptides in the samples were analyzed by mass *** analysis was performed to identify differentially expressed proteins and predict their biological functions.A total of 846 proteins were identified in the 21 samples,including 719 quantified *** total,49 significantly differentially expressed proteins(p0.05)were identified,including twenty-eight 0.2-fold up-regulated proteins or twenty-one 0.17-fold down-regulated *** up-regulated proteins,including cystic fibrosis transmembrane conductance regulator(CFTR)and carbonic anhydrase I(CAI),were of particular interest due to their role in regulating the oxygen saturation in deep *** Encyclopedia of Genes and Genomes(KEGG)pathway analysis indicated that these proteins were mainly involved in regulating actin cytoskeleton,local adhesion,biotin absorption and metabolism,immune system,cancer,and membrane transport *** conclusion,Tibetan middle-to-long distance runners who resided in high-altitude areas benefited from repeated plateau-plain alternate training mode during the pre-competition *** training mode induced positive changes in peripheral blood plasma proteins(CFTR and CAI