Identification and Expression Profile of a Neuropeptide LFRFamide-Like Gene During Different Stages of Gonadal Development in the Cephalopod Sepia pharaonis

作     者：QIU Jiayin SUN Lianlian LI Shuang ZHOU Xu CHI Changfeng ZHENG Libing QIU Jiayin;SUN Lianlian;LI Shuang;ZHOU Xu;CHI Changfeng;ZHENG Libing

作者机构：National and Provincial Joint Engineering Research Center for Marine Germplasm Resources Exploration and UtilizationSchool of Marine Science and TechnologyZhejiang Ocean UniversityZhoushan 316022China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2024年第23卷第2期

页      面：499-508页

核心收录：

学科分类：0908[农学-水产] 0707[理学-海洋科学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(No.31872547) the Natural Science Foundation of Zhejiang Province,China(No.LY20 C190007) the Open Foundation from Marine Sciences in the First-Class Subjects of Zhejiang(No.OFMS004) the Excellent Postdoctoral Program of Jiangsu Province(No.314865). 

主　　题：neuropeptide LFRFamide-like cephalopod cuttlefish Sepia pharaonis development reproduction 

摘      要：Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phylogenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four mature peptides at a concentration of 0.01μmol L^(−1) could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHOK1)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of S.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming.