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Journal of Ocean University of China 2024年第2期23卷 499-508页

作者： QIU Jiayin SUN Lianlian LI Shuang ZHOU Xu CHI Changfeng ZHENG Libing National and Provincial Joint Engineering Research Center for Marine Germplasm Resources Exploration and Utilization School of Marine Science and TechnologyZhejiang Ocean UniversityZhoushan 316022China

Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phylogenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four mature peptides at a concentration of 0.01μmol L^(−1) could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHOK1)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of S.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming.

关键词： neuropeptide LFRFamide-like cephalopod cuttlefish Sepia pharaonis development reproduction

Changes in embryonic development,juvenile growth and physiological adaptation of the cuttlefish Sepia pharaonis in response to photoperiod manipulation

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Journal of Oceanology and Limnology 2022年第5期40卷 2012-2027页

作者： Maowang JIANG Huan CHEN Shuangnan ZHOU Qingxi HAN Ruibing PENG Xiamin JIANG Key Laboratory of Applied Marine Biotechnology School of Marine SciencesNingbo UniversityNingbo 315211China

Photoperiod is a key environmental indicator for re gulating embryonic development,individual growth and physiological processes in aquatic animals.In this study,differences in embryonic development and performance of newly hatched cuttlefish juvenile exposed to five different cycles of light:dark(L:D):constant light,18 L:6D,12 L:12D,6 L:18D cycles,and constant darkness were evaluated.Prolonged exposure to light induced an accelerated rate of embryonic development,particularly after the red-bead stage.Principal component analysis(PCA)revealed that red-bead stage,heartbeat,endoskeleton formation,pigment appear,and six increments of cuttlebone were the main factors contributing to the embryonic development.Meaning that the duration time of these five stages were significantly different when exposed to photoperiod regimes,which may determine the duration of the incubation period of the embryos.Long term light has also affected the incubation parameters with an increased rate in hatching and shortened the incubation and hatching periods in the 12-24-h day length range.However,constant light and darkness environment appeared to have a greater effect on the stress of embryonic development,mainly reflected in the yolk shed ratio and the inking rate in the egg capsule.Moreover,the increase in the day length has contributed to improve the growth and survival of juveniles in the 12-18-h day length range;however,juveniles exposed to constant light and darkness experienced worse re sults in terms of growth,tissue glycogen content,dige stive enzymes of the digestive glands,and metabolic enzymes of the muscles.These finding suggest that prolonged light exposure accelerates the process of embryonic development,maximum feeding time is not necessarily a condition of optimal growth,and inappropriate light cycles can disturb the body’s endogenous controls.Therefore,the optimal photoperiod for the embryos development and juvenile growth of Sepia pharaonis were 12 h and 12-18 h of day length,respectively.These results are useful for

关键词： photoperiod embryonic development growth physiological changes cuttlefish

A Prototype of a Biomimetic Mantle Jet Propeller Inspired by cuttlefish Actuated by SMA Wires and a Theoretical Model for Its Jet Thrust

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Journal of Bionic Engineering 2014年第3期11卷 412-422页

作者： Fei Gao Zhenlong Wang Yukui Wang Yangwei Wang Jian Li State Key Laboratory of Robotics and System Harbin Institute of Technology Harbin 150001 Heilongjiang P. R. China

The cuttlefish have higher swimming speed and more maneuverability than most of the fish mainly benefiting from their unique jet propulsion mechanism, which is realized by the contraction and expansion of their flexible mantle. However it is difficult to mimic this jet propulsion mechanism using conventional electro-mechanical structures. In this paper, the musculature of the cuttlefish mantle and how the mantle flexibly contracts and expands were analyzed first. Then the Shape Memory Alloy（SMA） wires were chosen as the actuators and the soft silica gel was chosen as the body materials to develop a biomimetic mantle jet propeller. The SMA wires were embedded within the soft silica gel formed with cuttlefish mantle shape along the annular direction to mimic the circular muscles of cuttlefish mantle. The water was squeezed out the mantle cavity to form rear jets when the biomimetic mantle was contracted by SMA wires. A mechanical model and a thermal model were established to analyze the jet thrust and the jetting frequency. Theoretical analysis shows that the jet thrust is proportional to the square of the rate of change of SMA strain. Increasing the driving voltage can improve the rate of change of SMA strain, thus can improve both the jet thrust and the jetting frequency. However the j etting frequency is mainly restricted by the cooling of SMA wires. To maximize the jetting frequency, the optimal driving parameters for different driving voltage were calculated. The propulsion performance was tested and the results show that the jet thrust can increase with the driving voltage as predicted and the maximum average jet thrust is 0.14 N when the driving voltage is 25 V. The swimming test was carried out to verify the feasibility of the novel design. It is shown that the biomimetic jet propeller can swim with higher speed as the jet thrust and jetting frequency increase and the maximum speed can reach 8.76 cm·s^-1 （0.35 BL·s^-1） at a jetting frequency of 0.83 Hz.

关键词： cuttlefish biomimetic jet thrust shape memory alloy

Effects of Different Weaning Protocols on Survival,Growth and Nutritional Composition of Pharaoh cuttlefish(Sepia pharaonis)Juvenile

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Journal of Ocean University of China 2020年第6期19卷 1421-1429页

作者： JIANG Maowang HAN Ziru SHENG Peng PENG Ruibing HAN Qingxi JIANG Xiamin Key Laboratory of Applied Marine Biotechnology School of Marine SciencesNingbo UniversityNingbo 315211China

The aim of this study was to determine the optimal weaning protocols for rearing juvenile cuttlefish Sepia pharaonis,as well as to understand the feeding habits of cuttlefish and develop effective aqua-cultural practices.The effects of four experimental weaning protocols(i.e.,food type,cuttlefish size,food ration(bw d^−1)and feeding frequency(meals d^−1))on the growth,survival and muscle proximate composition of cuttlefish juvenile were studied in quintuplicate.The weaning period lasted 2 weeks.The results showed that cuttlefish fed with frozen white shrimp(Exopalaemon carinicauda)displayed a significantly higher survival,growth parameters,ingestion rate,food conversion rate,and muscle protein content when compared with the other prepared frozen foods.The larger individuals revealed better growth performance than smaller individuals,especially in terms of survival and specific growth rate,indicating that larger individuals were more receptive to frozen prey.Furthermore,size grading should be conducted to ensure better growth during weaning.As food rations increased,the survival,growth and muscle protein content of juveniles were promoted,whereas the most appropriate food ration for cuttlefish juvenile was 35%bw d^−1.Similarly,higher feeding frequency could stimulate the appetite of animals reared on weaning,and enhance survival,growth,and protein deposition compared to low feeding frequency.These results suggest that the optimal feeding protocols of juvenile weaning is as follows:the ideal prepared food is frozen shrimp,the weight of juveniles should be 4.0 g(32 days old)or more,the food ration should be 35%(bw d^−1),and the optimum feeding frequency is 4 meals day^-1.This study offers a promising starting point to successfully breed this candidate aquaculture species.

关键词： weaning protocols cuttlefish survival growth performance proximate composition

Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns

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Acta Oceanologica Sinica 2010年第6期29卷 121-124页

作者： WU Changwen CHI Changfeng HE Guangyuan LU Zhenming XU Meiying Key Laboratory of Molecular Biophysics Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory Huazhong University of Science and Technology Wuhan 430074 China Marine Science School Zhejiang Ocean University Zhoushan 316004 China Zhejiang Dahaiyang Science and Technology CO. Ltd. Zhoushan 316004 China

Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish,Sepiella maindroni.Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang,Guangdong province,China,in December 2007.The number of alleles ranged from 5 to 13.The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7,respectively,and the average polymorphic information content （P IC） was 0.778 5.These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S.maindroni.

关键词： SepieUa maindroni microsatellite DNA cuttlefish genetic variation

Distribution of Chitinolytic Enzymes in the Organs and cDNA Cloning of Chitinase Isozymes from the Liver of Golden cuttlefish <i>Sepia esculenta</i>

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Advances in Bioscience and Biotechnology 2017年第10期8卷 361-377页

作者： Ryo Nishino Hiromi Kakizaki Hideto Fukushima Masahiro Matsumiya Department of Marine Science and Resources College of Bioresource Sciences Nihon University Fujisawa Kanagawa Japan

The distribution of chitinolytic enzymes in eight organs of the golden cuttlefish Sepia esculenta was determined. Chitinase activity (activity of endo-type chitinolytic enzyme) was measured using pNP-(GlcNAc)n (n = 2, 3) as substrates, with high activity detected in the liver, posterior salivary gland, and stomach. β-N-acetylhexosaminidase (Hex) activity (activity of exo-type chitinolytic enzyme) was determined using pNP-(GlcNAc) as a substrate, and high activity was observed in six organs, including the liver, branchial heart, posterior salivary gland, and stomach. In addition, two chitin-binding proteins (CBP-A, CBP-B) were isolated from the liver using a chitin affinity column. Two full-length cDNAs (SeChi-1: 1484 bp;SeChi-2: 1748 bp) encoding chitinases were obtained from the liver of S. esculenta. SeChi-1 contained a 1377-bp open reading frame (ORF) encoding 459 amino acids, and SeChi-2 contained a 1656-bp ORF encoding 552 amino acids. Domain structures predicted from the deduced amino acid sequences of SeChi-1 and SeChi-2 (SeChi-1, SeChi-2) contained signal peptides, a GH Family 18 catalytic domain, one chitin binding domain (CBD) in SeChi-1, and two CBDs in SeChi-2. Proteome analysis revealed that 125 peptide residues of CBP-A were present in SeChi-1, and 116 peptide residues of CBP-B were present in SeChi-2. Organ expression analysis revealed that SeChi-1 and SeChi-2 were expressed only in the liver of S. esculenta. Phylogenetic analysis of SeChi-1, SeChi-2, and GH family 18 chitinases revealed that SeChi-2 belongs to a group of previously reported squid chitinases, while SeChi-1 does not belong to any previously reported group of mollusk chitinases.

关键词： Chitinolytic Enzyme Chitinase Distribution cDNA Cloning Golden cuttlefish Sepia esculenta

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Ten cuttlefish

中国多媒体与网络教学学报（电子版） 2019年第8X期 17-19,28页

作者：曾沛权广州市番禺区石基镇前锋小学

【案例概述】Ten cuttlefish是'攀登英语阅读系列分级阅读第一级'的一本故事类读物,讲述了Ten cuttlefish为了降温,把云喷黑让其下雨的神奇故事。故事情节巧妙离奇,能有效激发了学生的好奇心和想象力。本节课通过绘本阅读,培养和激发学... 详细信息

【案例概述】Ten cuttlefish是'攀登英语阅读系列分级阅读第一级'的一本故事类读物,讲述了Ten cuttlefish为了降温,把云喷黑让其下雨的神奇故事。故事情节巧妙离奇,能有效激发了学生的好奇心和想象力。本节课通过绘本阅读,培养和激发学生的想象力和创意思维。课前利用电子书包前置学习新词发音、感知词义,保障绘本阅读的连贯性;Free talk则为导入阅读文本服务。

关键词：绘本阅读阅读动机激发学生电子书包阅读素材小组讨论提高阅读能力情景导入 Jigsaw Ten cuttlefish

Determination of fatty acids percentages and profile extracted from cuttlefish of Iranian coasts of Persian Gulf and Oman Sea

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Journal of Coastal Life Medicine 2014年第11期2卷 855-858页

作者： Yosief Ali Asadpour Iranian Artemia Research Center Golmankhaneh harborUrmiaIran

Objective:To determine the fatty acid profile extracted from cuttlefish of Persian Gulf and Oman Sea,including Ancistrocheirus,Enoploteuthidae,Cranchiidae,Ommastrephidae and Loliginidae.Methods:Oil was extracted by the Bligh and Dyer method.The fatty acid profile of the oil was determined by gas chromatography.Results:The results showed that(13±5)%of wet weight of cuttlefish is oil.The results also showed that cuttlefish oil has 29.40%saturated fatty acids and 23.70%single-band unsaturated fatty acids,and the total value of the unsaturated multiple-band is 40.20%,the contents of arachidonic acid 2.78%,linolenic acid 3.10%,linoleic acid 5.20%,docosahexaenoic acid 15.40%,and eicosapentaenoic acid 9.60%out of the total fatty acids of the same may be mentioned.Conclusions:The results of the current study described that cuttlefish is considered for the first time as the new and rich source of omega-3 and omega-6.

关键词： Fatty acids cuttlefish Persian Gulf Oman Sea

Call it sleep-what animals without backbones can tell us about the phylogeny of intrinsically generated neuromotor rhythms during early development

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Neuroscience Bulletin 2013年第3期29卷 373-380页

作者： Michael A. Corner Netherlands Institute for Brain Research Amsterdam The Netherlands Université de Basse Normandie Caen France Harmoniehof 16/i 1071TC Amsterdam The Netherlands

A comprehensive overview is presented of the literature dealing with the development of sleep-like motility and neuronal activity patterns in non-vertebrate animals. It has been established that spontaneous, periodically modulated, neurogenic bursts of movement appear to be a universal feature of prenatal behavior. New empirical data are presented showing that such ＇seismic sleep＇ or ＇rapid-body-movement＇ bursts in cuttlefish persist for some time after birth. Extensive ontogenetic research in both vertebrates and non-vertebrates is thus essential before current hypotheses about the phylogeny of motorically active sleep-like states can be taken seriously.

关键词： sleep phylogeny behavior development spontaneous motility neuronal networks cuttlefish

Phylogeny of the cuttlefishes (Mollusca: Cephalopoda) based on mitochondrial COI and 16S rRNA gene sequence data

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Acta Oceanologica Sinica 2004年第4期23卷 699-707页

作者： LINXiangzhi ZHENGXiaodong XIAOShu WANGRucai MaricultureResearchLaboratory FisheryCollegeOceanUniversityofChinaQingdao266003China／／GraduateSchooloftheChineseAcademyofSciencesBeijing100039China MaricultureResearchLaboratory FisheryCollegeOceanUniversityofChinaQingdao266003China

To clarify cuttlefish phylogeny, mitochondrial cytochrome c oxidase subunit I (COI) gene and partial 16S rRNA geneare sequenced for 13 cephalopod species. Phylogenetic trees are constructed, with the neighbor-joining method.Coleoids are divided into two main lineages, Decabrachia and Octobrachia. The monophyly of the order Sepioidea,which includes the families Sepiidae, Sepiolidae and Idiosepiidae , is not supported. From the two families ofSepioidea examined, the Sepiolidae are polyphyletic and are excluded from the order. On the basis of 16S rRNA andamino acid of COI gene sequences data, the two genera (Sepiella and Sepia) from the Sepiidae can be distinguished, butdo not have a visible boundary using COI gene sequences. The reason is explained. This suggests that the 16S rDNAof cephalopods is a precious tool to analyze taxonomic relationships at the genus level, and COI gene is fitter at ahigher taxonomic level (i.e., family).

关键词： phylogeny cephalopods cuttlefish COI 16S rRNA