Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China

作     者：Jicheng Yu Liu Zhang Qiu Liu Xiaohui Qi Ying Ji Beom Seok Kim 

作者机构：College of Life Science Dalian Nationalities University College of Life Science and Biotechnology Korea University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2015年第5卷第7期

页      面：547-552页

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：Supported by the State Natural Sciences Foundation(No.31270057,No.21276047 and No.31070005) collaboration projects supported by Natural Science Foundation of China and Korea Research Foundation(No.31311140255 and No.2013K2A2A2000535) the Fundamental Research Funds for the Central Universities(DC201501020201) 

主　　题：Actinomycetes Antimicrobial activity 16S r RNA Yalujiang coastal wetland 

摘      要：Objective: To evaluate various types of samples from the different marine environments as sources of actinomycetes from the Yalujiang coastal wetland, North China, and to screen their antimicrobial properties. Further, the identified actinomycetes were characterized based on morphological, biochemical, and physiological ***: Eight different production media were used to isolate actinomycets from different stations of marine soil sediments in Yalujiang coastal wetland and the genotypic positions were established by 16 S r ***: A total of 172 actinomycetal isolates were obtained from 13 samples using five media. The most effective culture media in the isolation of actinobacteria were Gause s Synthetic agar and Starch-casein agar. Among 172 isolates, 46 isolates(26.74%) showed antibacterial activity, 70.93% belonged to the genus Streptomyces, others were Micromonospora spp. and Rhodococcus spp. Out of the 46 isolates, two cultures were further supported by morphological characterization ***: This is the first report about actinomycetes isolated from Yalujiang coastal wetland and it seems that the promising isolates from the unusual/unexplored wetland may prove to be an important step in the development of microbial natural product research.