Melittin enhanced intracellular trehalose with synergistic membrane stabilization of macromolecular protectants for cell cryopreservation

作     者：NIU QingJing GAO ShuHui ZHU KongYing REN LiXia YUAN XiaoYan 

作者机构：School of Materials Science and EngineeringTianjin Key Laboratory of Composite and Functional MaterialsTianjin UniversityTianjin 300350China Analysis and Measurement CenterTianjin UniversityTianjin 300072China 

出 版 物：《Science China(Technological Sciences)》 (中国科学（技术科学英文版）)

年 卷 期：2024年第67卷第4期

页      面：1160-1169页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

主　　题：melittin intracellular trehalose membrane stabilization macromolecular protectants cell cryopreservation 

摘      要：Cryopreservation plays an essential role in biobanking and cell therapy,but the physiological toxicity of traditional cryoprotectants such as glycerol and dimethyl sulfoxide(DMSO)has raised safety issues for biomedical ***,a nonreducing disaccharide that accumulates in desiccation-or cold-tolerant organisms,has been considered as a biocompatible ***,a naturally occurring membrane-active cationic peptide,melittin,was utilized to facilitate membraneimpermeable trehalose entry into cells for effective cell ***(ethylene glycol)and poly(vinyl pyrrolidone)were applied as macromolecular protectants to improve the stabilization of cell *** the optimal protocol,the postthaw recovery of human red blood cells in freezing bags at a hematocrit of~50%could achieve 82.9%with favorable cell morphologies and physiological ***,the cryosurvival of L929 fibroblasts reached 84.3%compared to the conventional method using 10%(v/v)*** short,this work by using trehalose and melittin provides a biocompatible solvent-free approach for long-term cryostorage of cells.