Dynamics Study on the Changes of Structure and Function of Erythrocyte Plasma Membrane under Different Storage Conditions

作     者：QUE Qing-he CHEN Wei-mei ZHONG Chang-rui QUE Qing-he;CHEN Wei-mei;ZHONG Chang-rui

作者机构：Longyan First Affiliated Hospital of Fujian Medical UniversityLongyan Fujian 364000China 

出 版 物：《Chinese Journal of Biomedical Engineering(English Edition)》 (中国生物医学工程学报（英文版）)

年 卷 期：2023年第32卷第3期

页      面：116-123页

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

主　　题：red blood cells cell membrane cytoplasm storage condition cell function 

摘      要：Objective:Red blood cells are the predominant blood cell component utilized in transfusion ***,prolonged storage and the buildup of metabolites can lead to alterations in the structure and function of the plasma membrane of RBCs,thereby impacting their *** study aimed to comprehensively examine the evolving dynamics of erythrocyte plasma membrane structure and function under varying storage ***:A two-factor,three-level experimental design was employed to investigate the impact of storage temperature(-4℃,4℃,and 10℃)on the composition of erythrocyte membrane *** performance liquid chromatography was utilized to analyze the phospholipid composition at 1,7,and 14 d of ***,the osmotic fragility of the erythrocyte membrane was assessed using the NaCl solution induction method,and the effective oxygen carrying capacity and oxygen affinity were determined using a blood oxygen ***:The findings indicated that the integrity of erythrocyte membranes could be adequately preserved at a temperature of-4℃.In contrast to the groups stored at 4℃and 10℃,there were no notable alterations in the phospholipid composition of erythrocyte membranes and related functional indicators at various time points in the-4℃*** erythrocyte membrane osmotic fragility in the-4℃group was(0.49±0.05)%,indicating sustained lower levels and suggesting the effective maintenance of cell membrane *** erythrocytes at-4℃did not have any detrimental impact on their ability to transport oxygen,as evidenced by the maintenance of normal effective oxygen carrying capacity(29.08±1.59)ml and oxygen affinity(5.33±0.48)***:The findings indicate that erythrocytes can be stored at-4℃for an extended period,with shorter stability observed at 4℃and 10℃.These results offer insights for optimizing the storage duration of erythrocyte products for specific ***,this research holds relevance for